Mitochondrial Small Heat Shock Proteins Are Essential for Normal Growth of Arabidopsis thaliana.
Mitochondrial Small Heat Shock Proteins Are Essential for Normal Growth of Arabidopsis thaliana.
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DOI：
10.3389/fpls.2021.600426
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发表时间：
2021
影响因子：
5.6
通讯作者：
Valle EM
中科院分区：
文献类型：
--
作者：
Escobar MR;Feussner I;Valle EM
线粒体在植物的逆境反应和电子传递链中产生的活性氧解毒中起着重要的作用。编码应激相关蛋白（如线粒体小热休克蛋白（M-sHSP））的基因表达在不同的非生物应激下上调。在拟南芥（Arabidopsis thaliana）中，已鉴定出3个M-sHSPs同源基因，尽管它们在生理条件下的功能尚不清楚。这项工作的目的是揭示所有三种M-sHSPs在整个植物水平上的体内功能。为了实现这一目标，我们分析了拟南芥M-sHSPs敲低系（单、双、三重敲低系）在正常植物生长期间的表型、蛋白质组学和代谢谱。在没有任何外源胁迫的情况下，三次敲落植株在营养和生殖阶段的表型变化最为显著。它们的叶片褪绿，生长停滞，种子产量低。同时，他们表现出糖、脯氨酸、柠檬酸、苹果酸和抗坏血酸以及其他代谢物水平的增加。相比之下，单敲和双敲植株在表型上表现出一些变化。三个M-sHSPs之间的冗余功能表明，与所有三个M-sHSPs基因同时丢失相关的营养和生殖生长损伤。三敲低系与对照植株相比，主要与光合作用和抗氧化防御有关的蛋白发生了改变。另一方面，热胁迫引发了不同的细胞质反应模式和其他sHSP成员的上调，在敲除植物中。总的来说，这三种M-sHSPs的耗尽严重影响了拟南芥的基本代谢过程，导致植物正常生长发育的改变。这些发现扩大了我们对细胞器特异性M-sHSPs在非胁迫条件下对植物健康生长的贡献的认识。
Mitochondria play important roles in the plant stress responses and the detoxification of the reactive oxygen species generated in the electron transport chain. Expression of genes encoding stress-related proteins such as the mitochondrial small heat shock proteins (M-sHSP) is upregulated in response to different abiotic stresses. In Arabidopsis thaliana, three M-sHSPs paralogous genes were identified, although their function under physiological conditions remains elusive. The aim of this work is to uncover the in vivo function of all three M-sHSPs at the whole plant level. To accomplish this goal, we analyzed the phenotype, proteomic, and metabolic profiles of Arabidopsis knock-down lines of M-sHSPs (single, double, and triple knock-down lines) during normal plant growth. The triple knock-down plants showed the most prominent altered phenotype at vegetative and reproductive stages without any externally applied stress. They displayed chlorotic leaves, growth arrest, and low seed production. Concomitantly, they exhibited increased levels of sugars, proline, and citric, malic, and ascorbic acid, among other metabolites. In contrast, single and double knock-down plants displayed a few changes in their phenotype. A redundant function among the three M-sHSPs is indicated by the impairment in vegetative and reproductive growth associated with the simultaneous loss of all three M-sHSPs genes. The triple knock-down lines showed alteration of proteins mainly involved in photosynthesis and antioxidant defense compared to the control plants. On the other hand, heat stress triggered a distinct cytosolic response pattern and the upregulation of other sHSP members, in the knock-down plants. Overall, depletion of all three M-sHSPs in Arabidopsis severely impacted fundamental metabolic processes, leading to alterations in the correct plant growth and development. These findings expand our knowledge about the contribution of organelle-specific M-sHSPs to healthy plant growth under non-stress conditions.
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影响因子：
3.7
作者：
Dabbaghizadeh A;Morrow G;Amer YO;Chatelain EH;Pichaud N;Tanguay RM
通讯作者：
Tanguay RM
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7.2
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影响因子：
11.6
作者：
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通讯作者：
Gillaspy, Glenda E.