Immunocytochemical and ultrastructural organization of the taste thalamus of the tree shrew (Tupaia belangeri).
Immunocytochemical and ultrastructural organization of the taste thalamus of the tree shrew (Tupaia belangeri).
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DOI：
10.1002/cne.25109
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发表时间：
2021-07-01
期刊：
影响因子：
--
通讯作者：
Erisir A
中科院分区：
文献类型：
--
作者：
Maher EE;Prillaman ME;Keskinoz EN;Petry HM;Erisir A
灵长类丘脑的丘脑后内侧核(VPMP)接受孤束核的直接输入，而啮齿类动物的同源丘脑结构则不接受。为了揭示这些核团中的突触电路是否有助于证明味觉丘脑在不同物种或一般情况下跨感觉丘脑的设计原则的保守性，我们表征了灵长类近亲树鼠(Tupaia Belangeri)VPMP的超微结构和分子特性，并将这些特性与啮齿类动物的味觉丘脑和哺乳动物的视觉丘脑的已知特性进行了比较。对VPMP内突触输入进行分类的电子显微镜分析显示，最大的终末含有许多小泡，并在多个中等口径的树突节段上形成大的突触后密度较高的突触带。有些在肾小球排列内形成三联体。较小的终末含有黑暗的线粒体；大多数在小直径的树突上形成单一的不对称或对称突触。免疫-EM实验表明，大型终末含有VGLUT2，而小型终末群含有VGLUT1或ChAT。这些发现提供了树鼠VPMP突触回路的形态和分子特征与非化学感觉丘脑核团相似的证据。此外，结果表明，在高等哺乳动物中，丘脑的所有初级感觉核都共享一个处理丘脑皮质感觉信息的结构模板。相比之下，啮齿动物和树鼠味觉核在形态和分子上的显著差异表明，这两个物种在味觉输入的细胞处理机制上存在根本差异。丘脑初级感觉核团的突触回路已经被很好地描述，并且在终末形态和化学特征上都表现出惊人的相似性。味觉丘脑是个例外，即VPMP。过去对大鼠味觉丘脑(VPMPC)突触回路的研究表明，它与LGN和MGN等其他核团有显著不同。我们的数据表明，树鼠VPMP具有与丘脑初级感觉核团相同的突触特征，包括突触前神经元间树突、大的VGluT2阳性终末和肾小球内的三叉神经排列。
Ventroposterior medialis parvocellularis (VPMP) nucleus of the primate thalamus receives direct input from the nucleus of the solitary tract, whereas the homologous thalamic structure in the rodent does not. To reveal whether the synaptic circuitries in these nuclei lend evidence for conservation of design principles in the taste thalamus across species or across sensory thalamus in general, we characterized the ultrastructural and molecular properties of the VPMP in a close relative of primates, the tree shrew (Tupaia belangeri), and compared these to known properties of the taste thalamus in rodent, and the visual thalamus in mammals. Electron microscopy analysis to categorize the synaptic inputs in the VPMP revealed that the largest-size terminals contained many vesicles and formed large synaptic zones with thick postsynaptic density on multiple, medium-caliber dendrite segments. Some formed triads within glomerular arrangements. Smaller-sized terminals contained dark mitochondria; most formed a single asymmetric or symmetric synapse on small-diameter dendrites. Immuno-EM experiments revealed that the large-size terminals contained VGLUT2, whereas the small-size terminal populations contained VGLUT1 or ChAT. These findings provide evidence that the morphological and molecular characteristics of synaptic circuitry in the tree shrew VPMP are similar to that in non-chemical sensory thalamic nuclei. Furthermore, the results indicate that all primary sensory nuclei of the thalamus in higher mammals share a structural template for processing thalamocortical sensory information. In contrast, substantial morphological and molecular differences in rodent versus tree shrew taste nuclei suggest a fundamental divergence in cellular processing mechanisms of taste input in these two species. The synaptic circuitry of primary sensory thalamic nuclei has been well characterized and show striking similarities both in terminal morphology and chemical characteristics. The exception to this is the taste thalamus, the VPMP. A past study of synaptic circuitry in the rat taste thalamus (VPMpc) revealed marked differences from other nuclei like the LGN and MGN. Our data demonstrates that the tree shrew VPMP shares synaptic characteristics common to primary sensory thalamic nuclei including presynaptic interneuron dendrites, large VGluT2 positive terminals, and triadic arrangements in glomeruli.
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DOI：
10.1002/cne.22223
发表时间：
2010-03-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Bickford ME;Slusarczyk A;Dilger EK;Krahe TE;Kucuk C;Guido W
通讯作者：
Guido W
影响因子：
0.7
作者：
Cao, J;Yang, EB;Chow, P
通讯作者：
Chow, P
影响因子：
2.5
作者：
Coomes, DL;Bickford, ME;Schofield, BR
通讯作者：
Schofield, BR
影响因子：
2.1
作者：
Agca, Selin;Houen, Gunnar;Trier, Nicole Hartwig
通讯作者：
Trier, Nicole Hartwig
影响因子：
2.5
作者：
CUCCHIARO, JB;UHLRICH, DJ;SHERMAN, SM
通讯作者：
SHERMAN, SM