Oxidative Stress Contributes to Bacterial Airborne Loss of Viability.
Oxidative Stress Contributes to Bacterial Airborne Loss of Viability.
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DOI：
10.1128/spectrum.03347-22
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发表时间：
2023-03-13
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
虽然空气中细菌活力的衰减已经观察了几十年，但对驱动衰减的机制的理解仍然难以捉摸。细菌的空气传播通常是其生命周期中的关键步骤，因此，表征驱动细菌空气传播衰变的机制是更全面了解微生物生态学的重要一步。使用受控电动悬浮和提取的生物气溶胶到基板（CELEBS），它是可能的，系统地评估不同的理化和环境参数对大肠杆菌的生存在空气中的Luria Bertani肉汤液滴的影响。而不是渗透胁迫驱动的活力损失，如最初认为的，氧化应激被发现发挥了关键作用。随着液滴蒸发并与周围环境平衡，表面积与体积比增加，这又增加了液滴中活性氧物质的形成。这些活性氧似乎在驱动E.杆菌细菌的空气传播具有广泛的影响，从疾病传播到云的形成。通过了解影响细菌在空气中稳定性的因素，我们可以更好地理解这些过程。然而，虽然我们几十年来已经知道空气中的细菌会逐渐丧失活力，但我们以前还没有确定驱动这一过程的机制。在这项工作中，我们发现空气中的液滴周围的氧气有助于液滴内活性氧的形成，然后逐渐破坏和杀死液滴内的细菌。这一发现表明，帮助细菌处理氧化应激的适应性也可能有助于它们在空气中的生存，并且是细菌空气传播病原体的必要适应性。了解细菌在空气中生存所需的适应性，最终可能会导致开发出旨在抑制其空气传播的新型抗菌剂，从而有助于预防疾病的传播。
While the airborne decay of bacterial viability has been observed for decades, an understanding of the mechanisms driving the decay has remained elusive. The airborne transport of bacteria is often a key step in their life cycle and as such, characterizing the mechanisms driving the airborne decay of bacteria is an essential step toward a more complete understanding of microbial ecology. Using the Controlled Electrodynamic Levitation and Extraction of Bioaerosols onto a Substrate (CELEBS), it was possible to systematically evaluate the impact of different physicochemical and environmental parameters on the survival of Escherichia coli in airborne droplets of Luria Bertani broth. Rather than osmotic stress driving the viability loss, as was initially considered, oxidative stress was found to play a key role. As the droplets evaporate and equilibrate with the surrounding environment, the surface-to-volume ratio increases, which in turn increased the formation of reactive oxygen species in the droplet. These reactive oxygen species appear to play a key role in driving the airborne loss of viability of E. coli. IMPORTANCE The airborne transport of bacteria has a wide range of impacts, from disease transmission to cloud formation. By understanding the factors that influence the airborne stability of bacteria, we can better understand these processes. However, while we have known for several decades that airborne bacteria undergo a gradual loss of viability, we have not previously identified the mechanisms driving this process. In this work, we discovered that oxygen surrounding an airborne droplet facilitates the formation of reactive oxygen species within the droplet, which then gradually damage and kill bacteria within the droplet. This discovery indicates that adaptations to help bacteria deal with oxidative stress may also aid their airborne survival and be essential adaptations for bacterial airborne pathogens. Understanding the adaptations bacteria need to survive in airborne droplets could eventually lead to the development of novel antimicrobials designed to inhibit their airborne survival, helping to prevent the transmission of disease.
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影响因子：
11
作者：
Bryan, N. C.;Christner, B. C.;Stewart, M. F.
通讯作者：
Stewart, M. F.
DOI：
10.1128/aem.13.5.781-787.1965
发表时间：
1965-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
HESS, GE
通讯作者：
HESS, GE
影响因子：
4.4
作者：
Fernandez, Mara Otero;Thomas, Richard J.;Reid, Jonathan P.
通讯作者：
Reid, Jonathan P.
使用气溶胶光学镊子和电动天平测量低相对湿度下过饱和氯化钠水溶液液滴的平衡尺寸
影响因子：
2.9
作者：
Hargreaves, G.;Kwamena, N. -O. A.;Reid, J. P.
通讯作者：
Reid, J. P.
DOI：
10.3390/antiox7050062
发表时间：
2018-04-27
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Gaucher C;Boudier A;Bonetti J;Clarot I;Leroy P;Parent M
通讯作者：
Parent M