All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse.
All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse.
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DOI：
10.1016/j.celrep.2023.112909
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发表时间：
2023-08-29
期刊：
影响因子：
8.8
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中科院分区：
文献类型：
--
作者：
确定神经代码的哪些特征驱动行为需要能够在许多神经元上同时高精度地读出和写入神经活动模式。结合双光子钙成像和靶向光刺激的全光学系统能够激活特定的、功能定义的神经元组。然而，这些技术无法测试整个群体的活动模式如何有助于计算，因为无法读取和写入细胞特定的放电率。为了克服这一挑战，我们取得了两项进展：首先，我们引入了一种小鼠遗传系，用于钙指示剂和强效体细胞靶向微生物视蛋白的 Cre 依赖性共表达。其次，利用这条线，我们开发了一种通过校准每个细胞的光刺激来读出和写入神经活动的精确群体向量的方法。这些进步为研究计算和行为的神经代码提供了一个强大而方便的平台。邦兹等人。开发并验证共同表达视蛋白和钙指示剂的 TIGRE2 转基因系，促进双光子全光学读/写实验。在这条线上，作者校准了每个目标细胞的全息光刺激，以准确地重建神经元群体的视觉诱发尖峰率。
Determining which features of the neural code drive behavior requires the ability to simultaneously read out and write in neural activity patterns with high precision across many neurons. All-optical systems that combine two-photon calcium imaging and targeted photostimulation enable the activation of specific, functionally defined groups of neurons. However, these techniques are unable to test how patterns of activity across a population contribute to computation because of an inability to both read and write cell-specific firing rates. To overcome this challenge, we make two advances: first, we introduce a genetic line of mice for Cre-dependent co-expression of a calcium indicator and a potent soma-targeted microbial opsin. Second, using this line, we develop a method for read-out and write-in of precise population vectors of neural activity by calibrating the photostimulation to each cell. These advances offer a powerful and convenient platform for investigating the neural codes of computation and behavior. Bounds et al. develop and validate a TIGRE2 transgenic line that co-expresses an opsin and calcium indicator, facilitating two-photon all-optical read/write experiments. In this line, the authors calibrate holographic photostimulation to each targeted cell to accurately recreate visually evoked spike rates across a population of neurons.
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影响因子：
16.2
作者：
Chen Y;Jang H;Spratt PWE;Kosar S;Taylor DE;Essner RA;Bai L;Leib DE;Kuo TW;Lin YC;Patel M;Subkhangulova A;Kato S;Feinberg EH;Bender KJ;Knight ZA;Garrison JL
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Garrison JL
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25
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Abdeladim, Lamiae
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Chen CC
影响因子：
7.7
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Baker, Christopher A.;Elyadat, Yishai M.;Bolton, M. McLean
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Bolton, M. McLean
影响因子：
25
作者：
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通讯作者：
Gradinaru V