1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing
1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing
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1A6/DRIM，人类 UTP20 在 28S 和 5.8S rRNA 加工中发挥作用
DOI：
10.1007/s11434-010-3166-8
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发表时间：
2010
影响因子：
--
通讯作者：
Yang Ke
中科院分区：
文献类型：
--
作者：
R. Kong;W. Han;H. Ulrich;T. Ning;Xiaojuan Du;Yang Ke
1A 6/DRIM是一种小亚基加工体组分，在18 SrRNA加工中起重要作用。在本研究中，当1A 6/DRIM在HeLa细胞中被沉默时，28 S rRNA和5.8S rRNA的成熟受到抑制;同时，观察到32 S rRNA前体的积累。用抗1A 6/DRIM抗体进行免疫沉淀，然后用ITS 2探针进行北方印迹。结果表明，1A 6/DRIM在体内与32 S和12 SrRNA前体结合。采用蔗糖密度梯度分离结合Western blot分析研究了1A 6/DRIM在rRNA加工过程中的表达谱。结果表明，1A 6/DRIM除了参与40 S前体外，还参与60 S前体的形成，并与32 S和12 SrRNA前体共同沉积在核仁中。此外，通过免疫沉淀揭示了U8 snoRNA与1A 6/DRIM的相互作用。这些结果表明1A 6/DRIM与32 SrRNA和U8 snoRNA都有相互作用，参与28 SrRNA和5.8SrRNA的加工。
Abstract1A6/DRIM has been identified as UTP20, a small subunit processome component, functioning in 18S rRNA processing. In the present study, the maturation of 28S rRNA and 5.8S rRNA was inhibited when 1A6/DRIM was silenced in HeLa cells; and coincidently, an accumulation of 32S rRNA precursor was observed. Immunoprecipitation was performed with the anti-1A6/DRIM antibody, followed by Northern blot with the ITS2 probe. The results showed that 1A6/DRIM was associated with both 32S and 12S rRNA precursors in vivo. The expression profile of 1A6/DRIM during rRNA processing was investigated by sucrose density gradient fractionation in combination with Western blot analysis. The results demonstrated that 1A6/DRIM was involved in the pre-60S particles in addition to the pre-40S particles and co-sediment with the 32S and 12S rRNA precursors in the nucleolus. Furthermore, the interaction of U8 snoRNA with 1A6/DRIM was revealed by immunoprecipitation. These results demonstrated that 1A6/DRIM interacted with both 32S rRNA and U8 snoRNA, being involved in 28S rRNA and 5.8S rRNA processing.
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