Local Treatment of Hydrogen-Rich Saline Promotes Wound Healing In Vivo by Inhibiting Oxidative Stress via Nrf-2/HO-1 Pathway.
Local Treatment of Hydrogen-Rich Saline Promotes Wound Healing In Vivo by Inhibiting Oxidative Stress via Nrf-2/HO-1 Pathway.
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富氢盐水的局部治疗通过 Nrf-2/HO-1 途径抑制氧化应激，促进体内伤口愈合
DOI：
10.1155/2022/2949824
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发表时间：
2022
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Li Y;Shen C;Zhou X;Zhang J;Lai X;Zhang Y
伤口愈合是一个复杂的动态过程，涉及大量的生物学事件。过度的氧化应激是延迟伤口愈合的关键因素。氢气是一种抗氧化、抗炎和抗凋亡的医用气体，具有安全、有效和渗透性。然而，局部处理氢对伤口愈合的影响及其潜在机制仍不清楚。本研究采用昆明种小鼠建立创伤模型。所有小鼠随机分为对照组、生理盐水局部治疗组、富氢盐水局部治疗组和富氢盐水腹腔注射组。为了评价富氢生理盐水对伤口愈合的影响，我们评估了伤口愈合率、伤口闭合时间、组织形态学、氧化应激指标、炎性细胞因子、凋亡指数和核因子-红细胞相关因子2（Nrf-2）的表达。此外，选择永生化的非致瘤性人表皮细胞（HaCaT），研究富氢培养基对氧化应激的治疗作用及其机制。结果表明，富氢生理盐水局部处理可缩短伤口闭合时间，降低促炎细胞因子和脂质过氧化水平。同时，降低细胞凋亡指数，增加Nrf-2表达。此外，富氢培养液通过激活Nrf-2/血红素加氧酶-1（HO-1）途径缓解了氧化应激。总之，富氢盐水局部治疗通过抗氧化、抗炎和抗凋亡作用表现出促进愈合的功能。氢通过Nrf-2/HO-1信号通路缓解创伤微环境中的氧化应激。本研究为促进创伤愈合提供了新的策略，为阐明创伤愈合机制提供了新的视角。
Wound healing is a complex dynamic process involving a large number of biological events. Excessive oxidative stress is a key factor delaying wound healing. Hydrogen is an antioxidant, anti-inflammatory, and antiapoptotic medical gas with safety, effectiveness, and penetrability. However, the effects of local treatment of hydrogen on wound healing and its potential mechanisms remain unclear. In this study, Kunming (KM) mice were used to set up a wound model. All the mice were randomly divided into the control, the local treatment with saline group, the local treatment with the hydrogen-rich saline group, and the intraperitoneal injection of the hydrogen-rich saline group. To evaluate the impact of hydrogen-rich saline on wound healing, we assessed the wound healing rate, wound closure time, histomorphology, oxidative stress indicators, inflammatory cytokines, the apoptosis index, and the expression of the nuclear factor-erythroid-related factor 2(Nrf-2). Furthermore, the immortalized nontumorigenic human epidermal (HaCaT) cells were chosen to investigate the therapeutic effects of hydrogen-rich medium on oxidative stress and its underlying mechanisms. The results showed that local treatment of hydrogen-rich saline shortened wound closure time and reduced the level of proinflammatory cytokines and lipid peroxidation. Meanwhile, it decreased the cell apoptosis index and increased the Nrf-2 expression. Besides, hydrogen-rich medium relieved the oxidative stress via the activation of the Nrf-2/heme oxygenase-1 (HO-1) pathway. In conclusion, local treatment of hydrogen-rich saline exhibits the healing-promoting function through antioxidant, anti-inflammatory, and antiapoptotic effects. Hydrogen relieves the oxidative stress in the wound microenvironment via Nrf-2/HO-1 signaling pathway. This study may offer a new strategy to promote wound healing and a new perspective to illustrate the mechanism of wound healing.
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