Beneficial effects of hydrogen-rich saline on early burn-wound progression in rats.
Beneficial effects of hydrogen-rich saline on early burn-wound progression in rats.
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富含氢的盐水对大鼠早期烧伤进展的有益影响。
DOI：
10.1371/journal.pone.0124897
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Han CM
中科院分区：
文献类型：
--
作者：
Guo SX;Jin YY;Fang Q;You CG;Wang XG;Hu XL;Han CM
深度烧伤伤口经历称为伤口进展的动态过程，其导致初始烧伤区域的加深和延伸。在存在灌注不足的情况下，在伤口进展期间，淤滞区更可能发展得更严重。据报道，氢可通过选择性淬灭氧自由基来减轻各种器官中由缺血/再灌注和烧伤引发的损伤。本研究的目的是探讨氢气对烧伤早期进展的可能保护作用。深度烧伤模型通过与煮沸的矩形黄铜梳子接触20 s建立。将五十六只Sprague-Dawley大鼠随机分为假手术组、烧伤+生理盐水组和烧伤+富氢生理盐水（HS）组。检测各组大鼠氧化应激、细胞凋亡和自噬指标。采用免疫荧光染色、ELISA、Western blot等方法对烧伤后瘀滞区进行评价，探讨烧伤后瘀滞区的变化及其机制。烧伤引起的丙二醛的增加显着减少与HS，而内源性抗氧化酶的活性显着增加。此外，HS治疗减弱烧伤后的细胞凋亡和自噬的增加，根据TUNEL染色结果和Bax，Bcl-2，caspase-3，Beclin-1和Atg-5蛋白的表达分析。此外，HS降低了瘀滞区的髓过氧化物酶水平和TNF-α、IL-1β和IL-6的表达，同时增加了IL-10。烧伤后Akt磷酸化水平升高，NF-κB p65表达下调。氢可以减缓深度烧伤后的早期伤口进展。氢气的有益作用是通过减轻氧化应激，抑制细胞凋亡和炎症，Akt/NF-κB信号通路可能参与调节炎性细胞因子的释放。
Deep burn wounds undergo a dynamic process known as wound progression that results in a deepening and extension of the initial burn area. The zone of stasis is more likely to develop more severe during wound progression in the presence of hypoperfusion. Hydrogen has been reported to alleviate injury triggered by ischaemia/reperfusion and burns in various organs by selectively quenching oxygen free radicals. The aim of this study was to investigate the possible protective effects of hydrogen against early burn-wound progression. Deep-burn models were established through contact with a boiled, rectangular, brass comb for 20 s. Fifty-six Sprague-Dawley rats were randomly divided into sham, burn plus saline, and burn plus hydrogen-rich saline (HS) groups with sacrifice and analysis at various time windows (6 h, 24 h, 48 h) post burn. Indexes of oxidative stress, apoptosis and autophagy were measured in each group. The zone of stasis was evaluated using immunofluorescence staining, ELISA, and Western blot to explore the underlying effects and mechanisms post burn. The burn-induced increase in malondialdehyde was markedly reduced with HS, while the activities of endogenous antioxidant enzymes were significantly increased. Moreover, HS treatment attenuated increases in apoptosis and autophagy postburn in wounds, according to the TUNEL staining results and the expression analysis of Bax, Bcl-2, caspase-3, Beclin-1 and Atg-5 proteins. Additionally, HS lowered the level of myeloperoxidase and expression of TNF-α, IL-1β, and IL-6 in the zone of stasis while augmenting IL-10. The elevated levels of Akt phosphorylation and NF-κB p65 expression post burn were also downregulated by HS management. Hydrogen can attenuate early wound progression following deep burn injury. The beneficial effect of hydrogen was mediated by attenuating oxidative stress, which inhibited apoptosis and inflammation, and the Akt/NF-κB signalling pathway may be involved in regulating the release of inflammatory cytokines.
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DOI：
10.1016/j.bbrc.2008.05.165
发表时间：
2008-08-15
影响因子：
3.1
作者：
Hayashida, Kentaro;Sano, Motoaki;Fukuda, Keiichi
通讯作者：
Fukuda, Keiichi
DOI：
10.1016/j.bbrc.2009.05.117
发表时间：
2009-08-14
影响因子：
3.1
作者：
Kajiya, Mikihito;Silva, Marcelo J. B.;Kawai, Toshihisa
通讯作者：
Kawai, Toshihisa
影响因子：
3.8
作者：
Ipaktchi, Kyros;Mattar, Aladdein;Arbabi, Saman
通讯作者：
Arbabi, Saman
影响因子：
2.2
作者：
Gravante, Gianpiero;Palmieri, Maria B.;Montone, Antonio
通讯作者：
Montone, Antonio
影响因子：
2.9
作者：
Eckermann JM;Chen W;Jadhav V;Hsu FP;Colohan AR;Tang J;Zhang JH
通讯作者：
Zhang JH