Automatic Microtitrator for Small Volume Samples.
Automatic Microtitrator for Small Volume Samples.
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DOI：
10.1021/acsmeasuresciau.2c00021
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发表时间：
2022-10-19
期刊：
影响因子：
--
通讯作者：
Papautsky, Ian
中科院分区：
文献类型：
--
作者：
Wu, Zhizhen;Rehman, Abid;Zhang, Zhehao;Papautsky, Ian
关键词：
用于护理点生物医学或使用点环境样品分析的电分析传感器由于检测限低、易于小型化、方便且能够处理小样品量而越来越受欢迎。由于必须严格控制 pH 值才能获得最佳电化学性能，因此通常需要调整这些样品的 pH 值。然而，手动滴定非常耗时，并且对于小体积来说尤其具有挑战性。终点确定也可能很困难。目前的商用自动pH滴定仪通常设计用于大体积（>1 mL）批量滴定，而现有的微量滴定仪充其量是半自动的，仍然依赖于多个手动步骤。为了解决这一问题，我们开发了一种适用于小体积样品的自动微量滴定系统。该系统使用消化后的全血微量样品进行了验证，成功证明了对小至 100 μL 样品的准确、快速的 pH 调节。该系统简单的模块化结构使其能够与痕量金属检测的酸洗以及其他清洁或样品制备步骤兼容。对血液中十亿分之一水平的锰重金属进行电化学检测表明，没有检测到系统引起的污染。最终，我们简单、准确、用户友好的自动微量滴定系统可用于微量样品的 pH 调节，并有可能扩展到其他 pH 终点分析。
Electroanalytical sensors for point-of-care biomedical or point-of-use environmental sample analysis are gaining popularity due to low limits of detection, ease of miniaturization, convenience, and ability to work with small sample volumes. Since pH must be tightly controlled for optimum electrochemical performance, adjustment of pH in these samples is often a necessity. Yet manual titration is time-consuming and can be especially challenging for small volumes. End point determination can also be difficult. Current commercial automatic pH titrators are generally designed for large volume (>1 mL) batch titrations, while the existing microvolume titrators are semiautomatic at best, still relying on multiple manual steps. To address the gap, we developed an automatic microtitration system suitable for small volume samples. The system was validated using digested whole blood microsamples, successfully demonstrating accurate and rapid pH adjustment for samples as small as 100 μL. The simple modular construction of the system makes it compatible with acid washing for trace metal detection and other cleaning or sample preparation steps. The electrochemical detection of manganese heavy metal in blood at the parts per billion level showed no detectable contamination induced by the system. Ultimately, our simple, accurate, user-friendly automatic microtitration system can be used in the pH adjustment of microvolume samples and can potentially be extended to other pH end point analysis.
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影响因子：
3
作者：
Jothimuthu, Preetha;Wilson, Robert A.;Herren, Josi;Pei, Xing;Kang, Wenjing;Daniels, Rodney;Wong, Hector;Beyette, Fred;Heineman, William R.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
2.8
作者：
Jothimuthu, Preetha;Wilson, Robert A.;Herren, Josi;Haynes, Erin N.;Heineman, William R.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
2.7
作者：
Chao, Chun-Tang;Sutarna, Nana;Wang, Chi-Jo
通讯作者：
Wang, Chi-Jo
影响因子：
3.4
作者：
HUTTON, RC
通讯作者：
HUTTON, RC
影响因子：
4.6
作者：
Kozak, Joanna;Paluch, Justyna;Koscielniak, Pawel
通讯作者：
Koscielniak, Pawel