Immunogenicity of the recombinant adenovirus fusion-expressing E0-E2 gene of the classical swine fever virus.
Immunogenicity of the recombinant adenovirus fusion-expressing E0-E2 gene of the classical swine fever virus.
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DOI：
10.3389/fmicb.2022.1054651
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发表时间：
2022
影响因子：
5.2
通讯作者：
Cao, Zhi
中科院分区：
文献类型：
--
作者：
Zhang, Heng;Yin, Dehua;Qin, Huairui;Zhang, Ke;Li, Zhaoyang;Cui, Guangchao;Ma, Guangbin;Sun, Peng;Cao, Zhi
关键词：
腺病毒载体疫苗一直是脑脊液疫苗的主流研究方向，由于腺病毒载体的复制缺陷，实现了灭活疫苗安全性和活疫苗有效性的双重效果。因此，本研究利用腺病毒载体表达E0和E2基因，构建E0-E2重组腺病毒疫苗（rAd-E0-E2），并确定最小免疫剂量和免疫持续时间。选用健康仔猪40头，随机分为8组，每组5头。第1 ~ 5组确定最小免疫剂量，5组分别接种不同免疫剂量的rAd-E0-E2。分别于免疫后7 d和14 d采集血清，采用ELISA法检测猪瘟抗体，并于免疫后7 d攻毒。第6 ~ 8组分别用1剂rAd-E0-E2、CSFV C株减毒活疫苗和生理盐水免疫，观察免疫持续期。在免疫后不同时间点采集血清，ELISA法检测猪瘟抗体，免疫后8个月攻毒。同时观察体温、临床症状及病理变化。结果1/5组攻毒后1头仔猪受保护，1/50剂量组有4头仔猪出现高热滞留、典型CSFV症状和组织病变，而1/10、1/5和1/5保护剂量组攻毒后均未观察到临床症状。最小剂量确定为1/10剂量。第6 ~ 8组试验结果表明，攻毒后所有仔猪均存活，但在免疫后8个月，rAd-E0-E2株的抗体水平高于C株，且阴性组仔猪攻毒后全部发病。总体而言，rAd-E0-E2的最小免疫剂量为1/10剂次（3.16 × 106.0 IFU），最小免疫剂量确定为1剂次（3.16 × 107.0 IFU），以达到预期效果。1剂rAd-E0-E2免疫仔猪的免疫持续期至少为8个月。
Adenovirus vector vaccines have been the mainstream research direction of CSF vaccines, due to the replication deficiency of adenovirus vectors, achieving double effects with the safety of inactivated vaccines and the efficacy of live vaccines. Therefore, the E0 and E2 genes were expressed by an adenovirus vector, a recombinant adenovirus E0-E2 (rAd-E0-E2) vaccine was constructed, and the minimum immunization dose and immune duration period were determined in this study. Forty healthy piglets were randomly divided into 8 groups (n = 5). Groups 1 ~ 5 were used to determine the minimum immunization dose, and 5 groups were inoculated with rAd-E0-E2 at different immune doses. Serum was collected at 7 d and 14 d after immunization to detect CSFV antibodies by ELISA, and piglets were challenged at 7 d post immunization. Groups 6 ~ 8 were immunized with 1 dose of rAd-E0-E2, the CSFV live attenuated vaccine C strain and saline to identify the immune duration period. Serum was collected at different time points after immunization, CSFV antibodies were detected by ELISA, and piglets were challenged at 8 months post immunization. Meanwhile, temperature, clinical symptoms and pathology were observed. The results of groups 1 ~ 5 showed that 1 piglet was protected after challenge, and 4 piglets exhibited high fever retention, typical CSFV symptoms and tissue lesions in the 1/50 dose group, whereas no clinical symptoms were observed in the 1/10 dose, 1/5 dose or 1 dose groups with 5/5 protection after challenge. The minimum dose was determined as 1/10 dose. The results of groups 6 ~ 8 showed that all piglets survived after challenge, but the antibody level of the rAd-E0-E2 strain was higher than that of the C strain at 8 months post immunization, and all piglets in the negative group developed the disease process after challenge. Overall, the minimum immunization dose of rAd-E0-E2 was 1/10 dose (3.16 × 106.0 IFU) and the minimum immune dose was determined to be 1 dose (3.16 × 107.0 IFU) to achieve the expected effects. The immune duration period of piglets immunized with 1 dose of rAd-E0-E2 was at least 8 months.
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DOI：
10.3390/v9040086
发表时间：
2017-04-21
期刊：
Viruses
影响因子：
--
作者：
Blome S;Staubach C;Henke J;Carlson J;Beer M
通讯作者：
Beer M
影响因子：
4.2
作者：
Xu, Huiling;Wang, Yanli;He, Fang
通讯作者：
He, Fang
影响因子：
2.4
作者：
Sun, Yuan;Liu, Da-Fei;Qiu, Hua-Ji
通讯作者：
Qiu, Hua-Ji
影响因子：
5.5
作者：
Gabitzsch, Elizabeth S.;Xu, Younong;Jones, Frank R.
通讯作者：
Jones, Frank R.
影响因子：
3.1
作者：
Xu, Xin-Gang;Chiou, Ming-Tang;Liu, Hung-Jen
通讯作者：
Liu, Hung-Jen