Salivary antigen SP32 is the immunodominant target of the antibody response to Phlebotomus papatasi bites in humans.
Salivary antigen SP32 is the immunodominant target of the antibody response to Phlebotomus papatasi bites in humans.
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DOI：
10.1371/journal.pntd.0001911
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发表时间：
2012
影响因子：
3.8
通讯作者：
Ben Ahmed M
中科院分区：
文献类型：
--
作者：
Marzouki S;Abdeladhim M;Abdessalem CB;Oliveira F;Ferjani B;Gilmore D;Louzir H;Valenzuela JG;Ben Ahmed M
Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is highly prevalent in Tunisia and is transmitted by a hematophagous vector Phlebotomus papatasi (P. papatasi). While probing for a blood meal, the sand fly injects saliva into the host's skin, which contains a variety of compounds that are highly immunogenic. We recently showed that the presence of anti-saliva antibodies was associated with an enhanced risk for leishmaniasis and identified the immunodominant salivary protein of Phlebotomus papatasi as a protein of approximately 30 kDa. We cloned and expressed in mammalian cells two salivary proteins PpSP30 and PpSP32 with predicted molecular weights close to 30 kDa from the Tunisian strain of P. papatasi. The two recombinant salivary proteins were purified by two-step HPLC (High-Performance Liquid Chromatography) and tested if these proteins correspond to the immunodominant antigen of 30 kDa previously shown to be recognized by human sera from endemic areas for ZCL and exposed naturally to P. papatasi bites. While recombinant PpSP30 (rPpSP30) was poorly recognized by human sera from endemic areas for ZCL, rPpSP32 was strongly recognized by the tested sera. The binding of human IgG antibodies to native PpSP32 was inhibited by the addition of rPpSP32. Consistently, experiments in mice showed that PpSP32 induced the highest levels of antibodies compared to other P. papatasi salivary molecules while PpSP30 did not induce any detectable levels of antibodies. Our findings demonstrate that PpSP32 is the immunodominant target of the antibody response to P. papatasi saliva. They also indicate that the recombinant form of PpSP32 is similar to the native one and represents a good candidate for large scale testing of human exposure to P. papatasi bites and perhaps for assessing the risk of contracting the disease. Leishmania is transmitted by female sand flies and deposited during a blood meal together with saliva. Saliva contains a vast repertoire of pharmacologically active molecules that facilitate the acquisition of the blood meal and contribute to the establishment of the infection. These molecules can induce the production of anti-saliva antibodies, which can be used as markers of exposure to the vector bite. Epidemiological studies using sand fly salivary gland extract as antigens are hampered by the difficulty in obtaining large amounts of salivary glands. In the present study, we have investigated the use of recombinant salivary proteins from the Tunisian strain of Phlebotomus papatasi, the main vector of zoonotic cutaneous leishmaniasis (ZCL) in Tunisia, as an alternative method for screening of exposure to the sand fly bites. We primarily identified PpSP32 as the immunodominant protein and described the low level of polymorphisms in the amino acid sequence of the target protein. Further, we tested the suitability of using the recombinant form of this protein to estimate positive anti-saliva antibodies in serum samples of individuals from an endemic area for ZCL. Our findings indicate that this protein represents a promising epidemiological tool that can aid in implementing control measures against leishmaniasis.
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影响因子：
4.4
作者：
Hostomská J;Volfová V;Mu J;Garfield M;Rohousová I;Volf P;Valenzuela JG;Jochim RC
通讯作者：
Jochim RC
影响因子：
2.4
作者：
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通讯作者：
Volf, P
影响因子：
2.8
作者：
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通讯作者：
Valenzuela, Jesus G.
DOI：
10.4269/ajtmh.2011.10-0598
发表时间：
2011-05-01
影响因子：
3.3
作者：
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通讯作者：
Louzir, Hechmi
影响因子：
2.1
作者：
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通讯作者：
Christensen, Bruce M.