Analysis of salivary transcripts and antigens of the sand fly Phlebotomus arabicus.
Analysis of salivary transcripts and antigens of the sand fly Phlebotomus arabicus.
复制标题
DOI：
10.1186/1471-2164-10-282
复制
发表时间：
2009-06-25
期刊：
影响因子：
4.4
通讯作者：
Jochim RC
中科院分区：
文献类型：
--
作者：
Hostomská J;Volfová V;Mu J;Garfield M;Rohousová I;Volf P;Valenzuela JG;Jochim RC
沙蝇唾液在血液摄食和利什曼原虫传播中起着重要作用，因为它被证明可以增加寄生虫的毒力。另一方面，对唾液成分的免疫会阻碍感染的建立。因此，最需要的是深入了解作为各种形式利什曼病载体的沙蝇物种的唾液组成。在目前的工作中，我们的重点是阿拉伯白蛉（Adlerius），最近被证明可以传播热带利什曼原虫，这是以色列皮肤利什曼病的病原体。构建了拟南芥雌性唾液腺cDNA文库，并对转录本进行了测序和分析。最丰富的蛋白家族是sp15样蛋白、parsp25样蛋白、d7相关蛋白、黄色相关蛋白、ppsp32样蛋白、抗原5相关蛋白和34个kda样蛋白。核酸内切酶、磷脂酶、焦磷酸酶、淀粉酶和海藻化酶等推测分泌酶的编码序列也被列出。质谱分析证实在唾液蛋白质组中存在20种预计分泌的蛋白质。研究了被arabicus咬伤的小鼠对唾液抗原的体液反应，并确定了许多唾液蛋白具有抗原性。本文对阿拉伯沙蝇唾液腺的转录组学分析是沙蝇亚属中唾液腺蛋白的首次描述。阿拉伯沙蝇唾液腺的蛋白质组学分析产生了迄今为止单一沙蝇物种中最全面的账户。提供了唾液蛋白的详细信息和系统发育关系，扩大了可能是沙蝇-宿主和沙蝇-利什曼原虫相互作用重要因素的分子知识库。酶学和免疫学研究进一步证明了功能转录组学在推进可能影响利什曼病的生物学和流行病学研究方面的价值。
Sand fly saliva plays an important role in blood feeding and Leishmania transmission as it was shown to increase parasite virulence. On the other hand, immunity to salivary components impedes the establishment of infection. Therefore, it is most desirable to gain a deeper insight into the composition of saliva in sand fly species which serve as vectors of various forms of leishmaniases. In the present work, we focused on Phlebotomus (Adlerius) arabicus, which was recently shown to transmit Leishmania tropica, the causative agent of cutaneous leishmaniasis in Israel. A cDNA library from salivary glands of P. arabicus females was constructed and transcripts were sequenced and analyzed. The most abundant protein families identified were SP15-like proteins, ParSP25-like proteins, D7-related proteins, yellow-related proteins, PpSP32-like proteins, antigen 5-related proteins, and 34 kDa-like proteins. Sequences coding for apyrases, hyaluronidase and other putative secreted enzymes were also represented, including endonuclease, phospholipase, pyrophosphatase, amylase and trehalase. Mass spectrometry analysis confirmed the presence of 20 proteins predicted to be secreted in the salivary proteome. Humoral response of mice bitten by P. arabicus to salivary antigens was assessed and many salivary proteins were determined to be antigenic. This transcriptomic analysis of P. arabicus salivary glands is the first description of salivary proteins of a sand fly in the subgenus Adlerius. Proteomic analysis of P. arabicus salivary glands produced the most comprehensive account in a single sand fly species to date. Detailed information and phylogenetic relationships of the salivary proteins are provided, expanding the knowledge base of molecules that are likely important factors of sand fly-host and sand fly-Leishmania interactions. Enzymatic and immunological investigations further demonstrate the value of functional transcriptomics in advancing biological and epidemiological research that can impact leishmaniasis.
登录
查看更多内容
影响因子：
2
作者：
Bahia, Diana;Gontijo, Nelder Figueiredo;Reis, Alexandre Barbosa
通讯作者：
Reis, Alexandre Barbosa
DOI：
10.1016/s0035-9203(03)00008-7
发表时间：
2004-01-01
影响因子：
2.2
作者：
Gebre-Michael, T;Balkew, M;Gramiccia, M
通讯作者：
Gramiccia, M
影响因子：
2.1
作者：
Dasgupta, Ranjit;Free, Heather M.;Christensen, Bruce M.
通讯作者：
Christensen, Bruce M.
影响因子：
3.8
作者：
Cerná, P;Mikes, L;Volf, P
通讯作者：
Volf, P
影响因子：
2.6
作者：
Elnaiem, DEA;Meneses, C;Lanzaro, GC
通讯作者：
Lanzaro, GC