Expression of epidermal keratins and filaggrin during human fetal skin development.
Expression of epidermal keratins and filaggrin during human fetal skin development.
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DOI：
10.1083/jcb.101.4.1257
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发表时间：
1985-10
影响因子：
7.8
通讯作者：
SUN, TT
中科院分区：
文献类型：
--
作者：
DALE, BA;HOLBROOK, KA;KIMBALL, JR;HOFF, M;SUN, TT
应用一维SDS PAGE和免疫印迹技术，对50多例人胚胎和胎儿皮肤上皮细胞的主要结构蛋白角蛋白和角蛋白细丝相关蛋白微丝蛋白进行了分析。配对标本进行免疫组织化学和电子显微镜检查。根据表皮的结构特征，可将人类表皮发育分为四个时期。第一个阶段是胚胎期(估计胎龄在9周之前)，其他阶段在胎儿期：层化(9-14周)、毛囊角化(14-24周)和毛囊间角化(大约24周开始)。酸性(AE1反应型，I型)和碱性(AE3反应型，II型)两个亚家族的角蛋白在整个发育过程中都存在。电子显微镜和免疫组织化学染色显示组织中存在角蛋白中间丝。角蛋白50kD和58kD在各年龄组(出生至出生)均有表达，56.5 kD和67kD角蛋白在层积时表达，并随着发育的进行而增加。40和52 kD的角蛋白存在于发育早期，但随着角质化而消失。免疫组织化学染色显示，从出生到复层，角蛋白50和58kD存在于基底层细胞，56.5和67kD存在于中间细胞，40和52kD存在于周皮和基底层细胞。微丝蛋白在大约15wk时首次被生化检测到，并通过免疫组织化学方法定位于毛囊周围的角化细胞。8-10wk后，在角化的毛囊间表皮的颗粒层和角化细胞层中发现了它。这些结果证明了以下几点。结构蛋白的表达与表皮发育过程中的形态变化密切相关。单个角蛋白的表达顺序与已知的角蛋白在单层和复层角化上皮中的表达顺序是一致的。复层上皮中典型的角蛋白(50和58kD)的表达先于层化，角化的典型角蛋白(56.5和67kD)的表达先于角化，这表明它们的表达标志着组织对这些过程的承诺。因为在胎儿发育过程中，只有在各种成人组织中发现的角蛋白才会表达，所以我们得出结论，并不存在“胎儿”角蛋白本身。
The major structural proteins of epithelia, the keratins, and the keratin filament-associated protein, filaggrin, were analyzed in more than 50 samples of human embryonic and fetal skin by one-dimensional SDS PAGE and immunoblotting with monoclonal and polyclonal antibodies. Companion samples were examined by immunohistochemistry and electron microscopy. Based on structural characteristics of the epidermis, four periods of human epidermal development were identified. The first is the embryonic period (before 9 wk estimated gestational age), and the others are within the fetal period: stratification (9-14 wk), follicular keratinization (14-24 wk), and interfollicular keratinization (beginning at approximately 24 wk). Keratin proteins of both the acidic (AE1-reactive, type I) and the basic (AE3-reactive, type II) subfamilies were present throughout development. Keratin intermediate filaments were recognized in the tissue by electron microscopy and immunohistochemical staining. Keratins of 50 and 58 kD were present in the epidermis at all ages studied (8 wk to birth), and those of 56.5 and 67 kD were expressed at the time of stratification and increased in abundance as development proceeded. 40- and 52-kD keratins were present early in development but disappeared with keratinization. Immunohistochemical staining suggested the presence of keratins of 50 and 58 kD in basal cells, 56.5 and 67 kD in intermediate cells, and 40 and 52 kD in the periderm as well as in the basal cells between the time of stratification and birth. Filaggrin was first detected biochemically at approximately 15 wk and was localized immunohistochemically in the keratinizing cells that surround hair follicles. It was identified 8-10 wk later in the granular and cornified cell layers of keratinized interfollicular epidermis. These results demonstrate the following. An intimate relationship exists between expression of structural proteins and morphologic changes during development of the epidermis. The order of expression of individual keratins is consistent with the known expression of keratins in simple vs. stratified vs. keratinized epithelia. Expression of keratins typical of stratified epithelia (50 and 58 kD) precedes stratification, and expression of keratins typical of keratinization (56.5 and 67 kD) precedes keratinization, which suggests that their expression marks the tissue commitment to those processes. Because only keratins that have been demonstrated in various adult tissues are expressed during fetal development, we conclude that there are no "fetal" keratins per se.(ABSTRACT TRUNCATED AT 400 WORDS)
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影响因子：
6.5
作者：
DALE, BA;STERN, IB;HUANG, LY
通讯作者：
HUANG, LY
影响因子：
64.8
作者：
DALE, BA;HOLBROOK, KA;STEINERT, PM
通讯作者：
STEINERT, PM
影响因子：
--
作者：
EPSTEIN, WL;MAIBACH, HI
通讯作者：
MAIBACH, HI
影响因子：
2.9
作者：
JACKSON, BW;GRUND, C;ILLMENSEE, K
通讯作者：
ILLMENSEE, K
影响因子：
2.7
作者：
FUKUYAMA, K;MARSHBURN, I;EPSTEIN, WL
通讯作者：
EPSTEIN, WL