Genomic Relatedness, Antibiotic Resistance and Virulence Traits of Campylobacter jejuni HS19 Isolates From Cattle in China Indicate Pathogenic Potential.
Genomic Relatedness, Antibiotic Resistance and Virulence Traits of Campylobacter jejuni HS19 Isolates From Cattle in China Indicate Pathogenic Potential.
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中国牛空肠弯曲菌 HS19 的基因组相关性、抗生素耐药性和毒力特征表明潜在的致病性
DOI：
10.3389/fmicb.2021.783750
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发表时间：
2021
影响因子：
5.2
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Zang X;Huang P;Li J;Jiao X;Huang J
虽然弯曲杆菌病是一种人兽共患食源性疾病，但动物来源的高危菌株很少有特征，人畜共患菌株的致病潜力仍然是有效干预人类感染的障碍。HS19已被公认为是以空肠弯曲菌为代表的标记型血清型，分离自感染后格林-巴利综合征(GBS)的患者空肠弯曲菌在发达国家流行。然而，先前的动物群体空肠弯曲菌分离株的血清型流行病学研究表明，HS19型在中国地区的牛分离株中也有流行。为了研究人畜共患病的潜在危害，本研究对14株牛HS19分离株进行了基因分型和表型分析。结果表明，这些牛分离株都属于ST-22复合体，在PubMLST数据库中来自世界各地患者的HS19临床分离物代表了77.2%的高危谱系，这表明ST-22复合体是HS19分离物的显著克隆复合体，以及HS19分离物在不同地区和宿主克隆传播的可能性。然而，这些牛的菌株与患者的HS19分离株聚在一起，表明了显著的系统发育亲缘关系和基因组相似性。重要的是，四环素基因tet(O)和gyrA(T86I)在牛分离株中的比例表达都高于在人类临床分离株中的比例。在所有牛分离株中都观察到了令人担忧的多药耐药(MDR)水平，在人类临床分离株中也存在两种牛分离株的MDR谱。值得注意的是，尽管35.7%的牛分离株与相同的HS19血清型和ST-22型序列相同，但仍有35.7%的牛分离株在IL-10-/-C57BL/6小鼠模型中引起严重的胃肠道病变，表明某些细菌可能因宿主适应而发生变化，从而引发疾病流行，因此相关的遗传因素值得进一步研究。本研究首次对牛HS19分离株进行了系统的细菌基因组学和体外毒力评价，加深了我们对具有高危血清型的食用动物分离株潜在的人畜共患病危害的认识，并为制定针对性的空肠弯曲菌缓解策略提供了关键信息。
Although campylobacteriosis is a zoonotic foodborne illness, high-risk isolates from animal sources are rarely characterized, and the pathogenic potential of zoonotic strains remains an obstacle to effective intervention against human infection. HS19 has been acknowledged as a maker serotype represented by Campylobacter jejuni (C. jejuni) isolates from patients with post-infection Guillain-Barré syndrome (GBS), which is circulation in developed countries. However, a previous serotype epidemiological study of C. jejuni isolates in an animal population revealed that HS19 was also prevalent in isolates from cattle in China. In this study, to investigate the hazardous potential of zoonotic strains, 14 HS19 isolates from cattle were systematically characterized both by genotype and phenotype. The results showed that all of these cattle isolates belonged to the ST-22 complex, a high-risk lineage represented by 77.2% HS19 clinical isolates from patients worldwide in the PubMLST database, indicating that the ST-22 complex is the prominent clonal complex of HS19 isolates, as well as the possibility of clonal spread of HS19 isolates across different regions and hosts. Nevertheless, these cattle strains clustered closely with the HS19 isolates from patients, suggesting a remarkable phylogenetic relatedness and genomic similarity. Importantly, both tetracycline genes tet(O) and gyrA (T86I) reached a higher proportional representation among the cattle isolates than among the human clinical isolates. A worrying level of multidrug resistance (MDR) was observed in all the cattle isolates, and two MDR profiles of the cattle isolates also existed in human clinical isolates. Notably, although shared with the same serotype HS19 and sequence type ST-22, 35.7% of cattle isolates induced severe gastrointestinal pathology in the IL-10–/– C57BL/6 mice model, indicating that some bacteria could change due to host adaptation to induce a disease epidemic, thus the associated genetic elements deserve further investigation. In this study, HS19 isolates from cattle were first characterized by a systematic evaluation of bacterial genomics and in vitro virulence, which improved our understanding of the potential zoonotic hazard from food animal isolates with high-risk serotypes, and provided critical information for the development of targeted C. jejuni mitigation strategies.
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影响因子：
5.2
作者：
Buchanan CJ;Webb AL;Mutschall SK;Kruczkiewicz P;Barker DOR;Hetman BM;Gannon VPJ;Abbott DW;Thomas JE;Inglis GD;Taboada EN
通讯作者：
Taboada EN
影响因子：
2.6
作者：
Lapierre, Lisette;Gatica, Maria A.;Vidal, Roberto
通讯作者：
Vidal, Roberto
影响因子：
14.2
作者：
Heikema, A. P.;Islam, Z.;Endtz, H. P.
通讯作者：
Endtz, H. P.
影响因子：
4.5
作者：
Guirado, Pedro;Paytubi, Sonia;Madrid, Cristina
通讯作者：
Madrid, Cristina
影响因子：
4.4
作者：
Habib, Ihab;Louwen, Rogier;De Zutter, Lieven
通讯作者：
De Zutter, Lieven