A handheld point-of-care genomic diagnostic system.
A handheld point-of-care genomic diagnostic system.
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DOI：
10.1371/journal.pone.0070266
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Lee LP
中科院分区：
文献类型：
--
作者：
Myers FB;Henrikson RH;Bone JM;Lee LP
快速检测和鉴定传染病病原体是发达国家和发展中国家卫生保健的一项关键需求。随着我们对病原体传染性和耐药性的基因组基础有了更多的了解，即时核酸检测可能会成为全球卫生的重要工具。在本文中，我们提出了一种廉价的、手持式的、电池供电的仪器，旨在使发展中国家的病原体基因分型成为可能。我们的微流体生物分子扩增阅读器（µBAR）代表了分子生物学，微流体，光学和电子技术的融合。µBAR能够进行等温核酸扩增分析，实时荧光读数，成本是传统台式热循环仪的一小部分。此外，µBAR还具有手机数据连接和GPS样本地理标记功能，可用于流行病学调查和远程医疗保健服务。µBAR通过集成电阻加热器控制分析温度，并使用led和光电晶体管监测来自60个单独反应室的实时荧光信号。试验在PDMS一次性微流体盒上进行，不需要外部电源进行样品加载。我们表征荧光检测限，加热器均匀性和电池寿命的仪器。作为原理证明，我们展示了利用环介导等温扩增（LAMP）实验，用µBAR检测HIV-1整合酶基因。虽然我们专注于检测纯化的DNA， LAMP之前已经在一系列临床样品中得到了证明，我们的最终目标是开发一种微流控装置，包括从原始样品中制备芯片上的样品。µBAR完全基于开源硬件和软件，在随附的在线补充中，我们提供了一整套原理图，物料清单，PCB布局，CAD图纸和µBAR仪器的源代码，旨在促进低成本基因诊断的进一步创新。
The rapid detection and identification of infectious disease pathogens is a critical need for healthcare in both developed and developing countries. As we gain more insight into the genomic basis of pathogen infectivity and drug resistance, point-of-care nucleic acid testing will likely become an important tool for global health. In this paper, we present an inexpensive, handheld, battery-powered instrument designed to enable pathogen genotyping in the developing world. Our Microfluidic Biomolecular Amplification Reader (µBAR) represents the convergence of molecular biology, microfluidics, optics, and electronics technology. The µBAR is capable of carrying out isothermal nucleic acid amplification assays with real-time fluorescence readout at a fraction of the cost of conventional benchtop thermocyclers. Additionally, the µBAR features cell phone data connectivity and GPS sample geotagging which can enable epidemiological surveying and remote healthcare delivery. The µBAR controls assay temperature through an integrated resistive heater and monitors real-time fluorescence signals from 60 individual reaction chambers using LEDs and phototransistors. Assays are carried out on PDMS disposable microfluidic cartridges which require no external power for sample loading. We characterize the fluorescence detection limits, heater uniformity, and battery life of the instrument. As a proof-of-principle, we demonstrate the detection of the HIV-1 integrase gene with the µBAR using the Loop-Mediated Isothermal Amplification (LAMP) assay. Although we focus on the detection of purified DNA here, LAMP has previously been demonstrated with a range of clinical samples, and our eventual goal is to develop a microfluidic device which includes on-chip sample preparation from raw samples. The µBAR is based entirely around open source hardware and software, and in the accompanying online supplement we present a full set of schematics, bill of materials, PCB layouts, CAD drawings, and source code for the µBAR instrument with the goal of spurring further innovation toward low-cost genetic diagnostics.
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影响因子：
14.2
作者：
Arnold, C;Westland, L;Gharbia, S
通讯作者：
Gharbia, S
DOI：
10.1088/0960-1317/18/6/067001
发表时间：
2008-06-01
影响因子：
2.3
作者：
Eddings, Mark A.;Johnson, Michael A.;Gale, Bruce K.
通讯作者：
Gale, Bruce K.
影响因子：
3.1
作者：
Hosaka N;Ndembi N;Ishizaki A;Kageyama S;Numazaki K;Ichimura H
通讯作者：
Ichimura H
影响因子：
6.1
作者：
Dimov, Ivan K.;Basabe-Desmonts, Lourdes;Lee, Luke P.
通讯作者：
Lee, Luke P.
影响因子：
6.1
作者：
Dimov, Ivan K.;Garcia-Cordero, Jose L.;Lee, Luke P.
通讯作者：
Lee, Luke P.