Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles.
Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles.
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DOI：
10.1371/journal.pone.0011535
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发表时间：
2010-07-12
期刊：
影响因子：
3.7
通讯作者：
Chute JP
中科院分区：
文献类型：
--
作者：
Meadows SK;Dressman HK;Daher P;Himburg H;Russell JL;Doan P;Chao NJ;Lucas J;Nevins JR;Chute JP
如果美国发生恐怖分子使用放射性或简易核武器进行的袭击，预计数十万人可能会受到危及生命的电离辐射。我们最近表明，外周血（PB）的全基因组表达分析可以生成基因表达谱，可以预测辐射暴露并区分全身照射（TBI）后的暴露剂量水平。然而，在发生大规模辐射伤亡事件时，由于部分屏蔽，许多受害者将受到异质暴露，并且尚不清楚 PB 基因表达谱是否有助于预测部分受辐射个体的状态。在这里，我们确定了 PB 中的基因表达谱，这些基因表达谱是 C57Bl6 小鼠前半体、后半体和单肢照射 0.5 Gy、2 Gy 和 10 Gy 的特征。与未受辐射的小鼠相比，这些 PB 特征能够以较高的准确度（范围 79-100%）预测部分受辐射小鼠的辐射状态。有趣的是，局部身体照射的 PB 特征很难预测损伤部位的辐射状态（范围 16-43%），这表明对局部身体照射的 PB 分子反应是解剖部位特异性的。重要的是，经 TBI 治疗的小鼠产生的 PB 基因特征完全无法预测部分受辐射动物或未受辐射对照的辐射状态。这些数据表明，局部身体照射，即使是单肢，也会产生辐射损伤的特征性 PB 特征，因此可能需要使用局部和全身的多个特征，以准确评估暴露于辐射的个体的状态。
In the event of a terrorist-mediated attack in the United States using radiological or improvised nuclear weapons, it is expected that hundreds of thousands of people could be exposed to life-threatening levels of ionizing radiation. We have recently shown that genome-wide expression analysis of the peripheral blood (PB) can generate gene expression profiles that can predict radiation exposure and distinguish the dose level of exposure following total body irradiation (TBI). However, in the event a radiation-mass casualty scenario, many victims will have heterogeneous exposure due to partial shielding and it is unknown whether PB gene expression profiles would be useful in predicting the status of partially irradiated individuals. Here, we identified gene expression profiles in the PB that were characteristic of anterior hemibody-, posterior hemibody- and single limb-irradiation at 0.5 Gy, 2 Gy and 10 Gy in C57Bl6 mice. These PB signatures predicted the radiation status of partially irradiated mice with a high level of accuracy (range 79–100%) compared to non-irradiated mice. Interestingly, PB signatures of partial body irradiation were poorly predictive of radiation status by site of injury (range 16–43%), suggesting that the PB molecular response to partial body irradiation was anatomic site specific. Importantly, PB gene signatures generated from TBI-treated mice failed completely to predict the radiation status of partially irradiated animals or non-irradiated controls. These data demonstrate that partial body irradiation, even to a single limb, generates a characteristic PB signature of radiation injury and thus may necessitate the use of multiple signatures, both partial body and total body, to accurately assess the status of an individual exposed to radiation.
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影响因子：
5.2
作者：
Fliedner, Theodor M.;Chao, Nelson J.;Bader, Judith L.;Boettger, Axel;Case, Cullen, Jr.;Chute, John;Confer, Dennis L.;Ganser, Arnold;Gorin, Norbert-Claude;Gourmelon, Patrick;Graessle, Dieter H.;Krawisz, Robert;Meineke, Viktor;Niederwieser, Dietger;Port, Matthias;Powles, Ray;Sirohi, Bhawna;Weinstock, David M.;Wiley, Albert;Coleman, C. Norman
通讯作者：
Coleman, C. Norman
影响因子：
3.7
作者：
Meadows, Sarah K.;Dressman, Holly K.;Muramoto, Garrett G.;Himburg, Heather;Salter, Alice;Wei, ZhengZheng;Ginsburg, Geoff;Chao, Nelson J.;Nevins, Joseph R.;Chute, John P.
通讯作者：
Chute, John P.
DOI：
10.2202/1544-6115.1436
发表时间：
2009-01-01
影响因子：
0.9
作者：
Lucas, Joseph;Carvalho, Carlos;West, Mike
通讯作者：
West, Mike
影响因子：
3.7
作者：
Lucas JE;Carvalho CM;Chen JL;Chi JT;West M
通讯作者：
West M
影响因子：
8.3
作者：
Baird, Alison E.
通讯作者：
Baird, Alison E.