A new nanosecond UV laser at 355 nm: early results of corneal flap cutting in a rabbit model.
A new nanosecond UV laser at 355 nm: early results of corneal flap cutting in a rabbit model.
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新型 355 nm 纳秒紫外激光：兔模型角膜瓣切割的早期结果
DOI：
10.1167/iovs.13-12580
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发表时间：
2013
影响因子：
4.4
通讯作者：
Reitsamer HA
中科院分区：
文献类型：
--
作者：
Trost A;Schrödl F;Strohmaier C;Bogner B;Runge C;Kaser-Eichberger A;Vogel A;Linz N;Freidank S;Hilpert A;Zimmermann I;Grabner G;Reitsamer HA
目的。方法：采用355nm紫外激光，激光波长为355nm (150khz，脉冲持续时间850 ps，光斑大小1 μm，光斑间距6× 6 μm，侧切Δz 1 μm，切深130 μm)，脉冲能量分别为2.2 μJ和2.5 μJ，制备角膜皮瓣（Chinchilla杂种，n= 25）。经裂隙灯检查，于治疗后6、12、24小时处死动物。结果：患者对激光治疗耐受良好，2.5 μJ比2.2 μJ更容易使角膜瓣提升；24小时的标准HE显示水平切口上皮完整，两能量下角膜厚度增加相似。无论能量水平如何，TUNEL分析显示水平和垂直切口在6、12和24小时的凋亡细胞数量相当，在24小时的水平切口中作为脱细胞间质带被检测到。超微结构分析显示角膜上皮和内皮细胞形态规则，而在基质中，可以检测到紊乱的胶原片层，代表水平切口，再次与应用的能量水平无关。结论：这种新的紫外激光在角膜皮瓣切割的能量范围内未发现角膜上皮和内皮细胞损伤。与现有的紫外线激光研究相比，观察到的角膜肿胀更低，尽管这里应用的总能量要高得多。观察到的间质角质形成细胞的损失与现有的激光系统相当。因此，这种新型激光器适用于屈光手术，等待在慢性环境下的测试。
Purpose.: A new 355 nm UV laser was used for corneal flap cutting in an animal model and tested for clinical and morphologic alterations.Methods.: Corneal flaps were created (Chinchilla Bastards; n= 25) with an UV nanosecond laser at 355 nm (150 kHz, pulse duration 850 ps, spot-size 1 μm, spot spacing 6× 6 μm, side cut Δz 1 μm; cutting depth 130 μm) and pulse energies of 2.2 or 2.5 μJ, respectively. Following slit-lamp examination, animals were killed at 6, 12, and 24 hours after treatment. Corneas were prepared for histology (hematoxylin and eosin [HE], TUNEL-assay) and evaluated statistically, followed by ultrastructural investigations.Results.: Laser treatment was tolerated well, flap lift was easier at 2.5 μJ compared with 2.2 μJ. Standard HE at 24 hours revealed intact epithelium in the horizontal cut, with similar increase in corneal thickness at both energies. Irrespective of energy levels, TUNEL assay revealed comparable numbers of apoptotic cells in the horizontal and vertical cut at 6, 12, and 24 hours, becoming detectable in the horizontal cut as an acellular stromal band at 24 hours. Ultrastructural analysis revealed regular morphology in the epi-and endothelium, while in the stroma, disorganized collagen lamellae were detectable representing the horizontal cut, again irrespective of energy levels applied.Conclusions.: This new UV laser revealed no epi-nor endothelial damage at energies feasible for corneal flap cutting. Observed corneal swelling was lower compared with existing UV laser studies, albeit total energy applied here was much higher. Observed loss of stromal keratinocytes is comparable with available laser systems. Therefore, this new laser is suitable for refractive surgery, awaiting its test in a chronic environment.
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影响因子：
3.3
作者：
DOUGHTY, MJ;CULLEN, AP
通讯作者：
CULLEN, AP
DOI：
10.3928/1081597x-20090917-08
发表时间：
2009-10
期刊：
Journal of refractive surgery (Thorofare, N.J. : 1995)
影响因子：
--
作者：
de Medeiros FW;Kaur H;Agrawal V;Chaurasia SS;Hammel J;Dupps WJ Jr;Wilson SE
通讯作者：
Wilson SE
320 nm以下的紫外线对兔子眼前节的损伤作用。
影响因子：
2.5
作者：
J. Čejková;Z. Lojda
通讯作者：
Z. Lojda
眼部光损伤
影响因子：
3.3
作者：
S. Zigman
通讯作者：
S. Zigman
紫外线暴露的兔角膜细胞凋亡
影响因子：
2.8
作者：
A. Podskochy;L. Gan;P. Fagerholm
通讯作者：
P. Fagerholm