Monoclonal antibody against mouse sperm blocks a specific event in the fertilization process.
Monoclonal antibody against mouse sperm blocks a specific event in the fertilization process.
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针对小鼠精子的单克隆抗体可阻断受精过程中的特定事件。
DOI：
10.1002/jez.1402270318
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
O'Rand,MG
中科院分区：
文献类型：
--
作者：
Saling,PM;Raines,LM;O'Rand,MG
将骨髓瘤细胞(NS-1/X63Ag8)与Balb/c雄性小鼠脾细胞进行融合，获得杂交瘤细胞系。以小鼠附睾尾精子的间接免疫荧光为初步筛选方法。这一程序允许立即鉴定产生与受精过程相关的单抗的杂交瘤。杂交瘤细胞系M29分泌一种IgM类抗体，定位于小鼠精子头的赤道部分。这种抗体不是物种特异性的，但仅限于所有其他被检查的精子(仓鼠、兔子、人)中的同源功能区，即赤道段。当测试对受精过程的可能影响时，含有M29抗体的腹水在体外非常有效地阻断小鼠配子的受精。无论M29处理的精子首先遇到的是卵丘细胞层、透明带还是卵子质膜，都观察到了相同的抑制活性。当存在这一层时，大量M29处理的精子能够穿透透明带。因此，M29单抗似乎在精子和卵子质膜之间的相互作用水平上特异性地阻止受精；其他受精前事件(过度激活的运动、小带结合、顶体反应、小带穿透)似乎几乎没有受到影响。使用针对特定生物事件的M29抗体和相关探针，应该可以从分子角度绘制精子细胞膜的功能区域图。
Hybridoma cell lines were produced by PEG (polyethylene glycol)‐induced fusion of myeloma cells (NS‐1/X63 Ag 8) with dispersed spleen cells from a Balb/c male mouse immunized syngeneically with homogenized Balb/c testis. Indirect immunefluorescence on mature (cauda epididymal) mouse sperm was employed as the primary screening assay. This procedure allowed the immediate identification of hybridomas which produce monoclonal antibodies of interest with respect to the fertilization process. One hybridoma cell line, M29, secretes an antibody of the IgM class that localizes to the equatorial segment of the mouse sperm head. This antibody is not species specific, but is restricted to the homologous functional area, the equatorial segment, in all other sperm examined (hamster, rabbit, human). When tested for a possible effect on the fertilization process, ascites fluid containing M29 antibody was very effective in blocking the fertilization of mouse gametes in vitro. The same inhibiting activity was observed regardless of whether the M29‐treated sperm first encountered the cumulus cell layer, the zona pellucida, or the egg plasma membrane. A large number of M29‐treated sperm were capable of penetrating zonae pellucidae when this layer was present. It appears, therefore, that M29 monoclonal antibody prevents fertilization specifically at the level of interaction between the sperm and egg plasma membranes; the other prefertilization events (hyperactivated motility, zona binding, acrosome reaction, zona penetration) seem little affected. Using the M29 antibody and related probes that are specific for particular biological events, it should be possible to map, in molecular terms, the functional domains of the sperm cell's membrane.
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影响因子：
2.5
作者：
NODA, YD;YANAGIMACHI, R
通讯作者：
YANAGIMACHI, R
大鼠卵子在体外通过附睾精子受精以及移植后卵子的发育。
DOI：
--
发表时间：
1974
期刊：
Journal of Reproduction and Fertility
影响因子：
--
作者：
Y. Toyoda;M. Chang
通讯作者：
M. Chang
影响因子：
4
作者：
C. Millette;D. A. O’Brien;C. Moulding
通讯作者：
C. Moulding
影响因子：
3.6
作者：
INOUE, M;WOLF, DP
通讯作者：
WOLF, DP
精子发生的 28K 道尔顿细胞表面自身抗原：使用单克隆抗体进行表征。
影响因子：
2.7
作者：
S. Gaunt
通讯作者：
S. Gaunt