Fluorescent nanoparticle-based indirect immunofluorescence microscopy for detection of Mycobacterium tuberculosis.
Fluorescent nanoparticle-based indirect immunofluorescence microscopy for detection of Mycobacterium tuberculosis.
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基于荧光纳米颗粒的间接免疫荧光显微镜检测结核分枝杆菌
DOI：
10.1155/2007/89364
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发表时间：
2007
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Qin D;He X;Wang K;Zhao XJ;Tan W;Chen J
建立了一种基于荧光纳米颗粒的间接免疫荧光显微镜(FNP-IIFM)快速检测结核分枝杆菌的方法。以抗结核分枝杆菌抗体为一抗识别结核分枝杆菌，然后用三(2，2-联吡啶)二氯六水合铀(II)掺杂的二氧化硅纳米颗粒标记的抗体结合蛋白(蛋白A)产生荧光信号用于显微镜检测。在检测前，蛋白A被固定在RuBpy掺杂的二氧化硅纳米颗粒上，覆盖∼为5.1×10~2个分子/纳米颗粒。用该方法可检测出细菌混合物中的结核分枝杆菌以及添加痰中的结核分枝杆菌。与直接使用常规荧光染料作为标记相比，使用荧光纳米颗粒显示出放大的信号强度和更高的光稳定性。我们的初步研究已经证明了FNP-IIFM方法在临床标本中快速检测结核分枝杆菌的潜在应用。
A method of fluorescent nanoparticle-based indirect immunofluorescence microscopy (FNP-IIFM) was developed for the rapid detection of Mycobacterium tuberculosis. An anti-Mycobacterium tuberculosis antibody was used as primary antibody to recognize Mycobacterium tuberculosis, and then an antibody binding protein (Protein A) labeled with Tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy)-doped silica nanoparticles was used to generate fluorescent signal for microscopic examination. Prior to the detection, Protein A was immobilized on RuBpy-doped silica nanoparticles with a coverage of ∼5.1×102 molecules/nanoparticle. With this method, Mycobacterium tuberculosis in bacterial mixture as well as in spiked sputum was detected. The use of the fluorescent nanoparticles reveals amplified signal intensity and higher photostability than the direct use of conventional fluorescent dye as label. Our preliminary studies have demonstrated the potential application of the FNP-IIFM method for rapid detection of Mycobacterium tuberculosis in clinical samples.
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DOI：
10.1164/ajrccm.161.5.9908125
发表时间：
2000-05-01
影响因子：
24.7
作者：
Chan, ED;Reves, R;Hahn, WE
通讯作者：
Hahn, WE
影响因子：
9.4
作者：
Park, H;Jang, HJ;Kim, C
通讯作者：
Kim, C
影响因子：
4.9
作者：
Santra, S;Yang, H;Mericle, RA
通讯作者：
Mericle, RA
影响因子：
3.5
作者：
Santra, S;Wang, KM;Tan, WH
通讯作者：
Tan, WH
影响因子：
7.4
作者：
Herr, Joshua K.;Smith, Joshua E.;Tan, Weihong
通讯作者：
Tan, Weihong