Digitized molecular detection on off-the-shelf Blu-ray discs: Upgraded resolution and enhanced sensitivity
Digitized molecular detection on off-the-shelf Blu-ray discs: Upgraded resolution and enhanced sensitivity
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现成蓝光光盘上的数字化分子检测：升级的分辨率和增强的灵敏度
DOI：
10.1016/j.snb.2016.11.042
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发表时间：
2017-04
期刊：
影响因子：
--
通讯作者：
Hua-Zhong Yu
中科院分区：
文献类型：
--
作者：
Maolin Shi;Samuel S. H. Weng;Xiaochun Li;Hua-Zhong Yu
除了存储和存档高清晰度电影和游戏的固有能力之外，现成的蓝光光盘已被用于制备分子结合分析，随后利用标准的计算机驱动器与光盘质量检查程序一起读取和定量。这种数字化的分子检测系统的性能已经首先用人造的“墨水分析”(一组微米大小的墨点)进行了检验，以确定定量能力和横向分辨率。用蓝光检测平台对常规的生物素-链霉亲和素结合试验进行了检测，并与DVD检测结果进行了比较。升级的横向分辨率(<；100μm)和增强的分析性能(试验生物素-链霉亲和素系统的线性响应高达0.4gμg/m，低密度估计为<；0.1μg/m)增加了其潜力，使其成为一种经济高效的定量诊断工具，用于现场分析和微量护理点医学诊断。
Beyond the intrinsic capability of storing and archiving high-definition movies and games, off-the-shelf Blu-ray discs have been adopted for the preparation of molecular binding assays, which are subsequently read and quantitated with a standard computer drive in conjunction with the disc-quality check program. The performance of this digitized molecular detection system has been examined first with an artificial “ink assay” (an array of microsize ink dots) to define the quantitation capability and the lateral resolution. The conventional biotin-streptavidin binding assay was then tested with the Blu-ray detection platform, and compared with the resultsobtained on a DVD. The upgraded lateral resolution (<100 μm) and enhanced assay performance (linear response up to 0.4 μg/mL and LOD estimated to be <0.1 μg/mL for the trial biotin-streptavidin system) augments its potential to be adapted as a cost-effective quantitative diagnostic tool for on-site analysis and point-of-care medical diagnosis at trace amounts.
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DOI：
--
发表时间：
2009-02
期刊：
--
影响因子：
--
作者：
J. Peek;J. Bergmans;J. Haaren;F. Toolenaar;S. Stan
通讯作者：
J. Peek;J. Bergmans;J. Haaren;F. Toolenaar;S. Stan
影响因子：
18.3
作者：
Hua-Zhong Yu;Yunchao Li;L. M. Ou
通讯作者：
Hua-Zhong Yu;Yunchao Li;L. M. Ou
影响因子：
8.4
作者：
Xue-Jiao Zhao;Xiaochun Li;C. Cui;Hua-Zhong Yu
通讯作者：
Xue-Jiao Zhao;Xiaochun Li;C. Cui;Hua-Zhong Yu
影响因子：
6.1
作者：
Xiaochun Li;S. Weng;B. Ge;Zhihui Yao;Hua-Zhong Yu
通讯作者：
Xiaochun Li;S. Weng;B. Ge;Zhihui Yao;Hua-Zhong Yu
影响因子：
12.6
作者：
Donolato, Marco;Antunes, Paula;Hansen, Mikkel Fougt
通讯作者：
Hansen, Mikkel Fougt