The adipokine C1q TNF related protein 3 (CTRP3) is elevated in the breast milk of obese mothers.
The adipokine C1q TNF related protein 3 (CTRP3) is elevated in the breast milk of obese mothers.
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作者：
Kwon MR;Cress E;Clark WA;Alamian A;Lu Y;Peterson JM
C1 q肿瘤坏死因子相关蛋白3（C1 q TNF related protein 3，CTRP 3）是一种相对较新的主要来源于脂肪组织的激素因子，具有抗糖尿病的特性。为了确定CTRP 3是否可以在儿童早期发育中发挥作用，本研究的目的是确定母乳（BM）中CTRP 3的存在，并确定CTRP 3水平是否与母亲的妊娠前肥胖状态相关。母乳采集自妊娠前体重指数（BMI）分类为正常体重（BMI 18-25 kg/m2，n = 23）或肥胖（BMI > 30 kg/m2，n = 14）的母乳喂养母亲。免疫沉淀，然后免疫印迹分析证实了CTRP 3在BM中的存在。通过ELISA测定BM样品中CTRP 3的浓度。另外的生物活性成分也通过市售测定法测量：生长素释放肽、胰岛素、瘦素、脂联素、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和葡萄糖。适当时，使用非配对t检验（参数）和Mann-Whitney U检验（非参数）比较正常体重和肥胖母亲的生物活性成分。这项研究的主要发现是脂肪因子CTRP 3存在于BM中，并且CTRP 3水平随着妊娠前肥胖而增加。此外，这项研究独立地证实了先前的工作，即肥胖母亲的BM具有更高浓度的胰岛素和瘦素。此外，肥胖和正常体重母亲之间的BM在ghrelin、脂联素、IL-6、TNF-α或葡萄糖水平方面没有观察到差异。这项研究确定了BM中的一种新因子CTRP 3，并表明肥胖母亲的BM CTRP 3水平较高。由于CTRP 3的胰岛素增敏作用，肥胖母亲BM中升高的CTRP 3水平可能抵消肥胖母亲BM中升高的瘦素和胰岛素水平的负面影响。未来的研究将需要进行，以确定CTRP 3在BM中的相关性，并检查其他脂肪组织来源的激素因子的存在。
C1q TNF related protein 3 (CTRP3) is a relatively novel hormonal factor primarily derived from adipose tissue and has anti-diabetic properties. To determine if CTRP3 could play a role in early childhood development, the purpose of this study was to establish the presence of CTRP3 in breast milk (BM) and to determine whether CTRP3 levels were correlated with pregravid obesity status of the mother. Breast milk was collected from breast-feeding mothers who had a pregravid body mass index (BMI) classification of normal weight (BMI 18–25 kg/m2, n = 23) or obese (BMI > 30 kg/m2, n = 14). Immunoprecipitation followed by immunoblot analysis confirmed the presence of CTRP3 in BM. The concentration of CTRP3 in BM samples was determined by ELISA. Additional bioactive components were also measured by commercially available assays: ghrelin, insulin, leptin, adiponectin, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glucose. Bioactive components in normal weight and obese mothers were compared using unpaired t-test (parametric) and Mann–Whitney U-test (non-parametric), as appropriate. The primary findings of this study are that the adipokine CTRP3 is present in BM and CTRP3 levels are increased with pregravid obesity. Additionally, this study independently confirmed previous work that BM from obese mothers has a higher concentration of insulin and leptin. Further, no differences were observed in BM between obese and normal weight mothers in ghrelin, adiponectin, IL-6, TNF-α, or glucose levels. This study identified a novel factor in BM, CTRP3, and showed that BM CTRP3 levels higher in obese mothers. Because of the purported insulin sensitizing effect of CTRP3, it is possible that the elevated levels of CTRP3 in the BM of obese mothers may offset negative effects of elevated leptin and insulin levels in the BM of obese mothers. Future studies will need to be conducted to determine the relevance of CTRP3 in BM and to examine the presence of other adipose tissue-derived hormonal factors.
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DOI：
10.1001/jama.2014.732
发表时间：
2014-02-26
期刊：
JAMA
影响因子：
--
作者：
Ogden CL;Carroll MD;Kit BK;Flegal KM
通讯作者：
Flegal KM
影响因子：
4.2
作者：
DelPrado, M;Delgado, G;Villalpando, S
通讯作者：
Villalpando, S
影响因子：
7.1
作者：
Ekelund, Ulf;Anderssen, Sigmund;Brage, Soren
通讯作者：
Brage, Soren
影响因子：
25.7
作者：
Oben, Jude A.;Mouralidarane, Angelina;Taylor, Paul D.
通讯作者：
Taylor, Paul D.
影响因子：
5
作者：
Harder, T;Bergmann, R;Plagemann, A
通讯作者：
Plagemann, A