Quantification of nanoscale density fluctuations by electron microscopy: probing cellular alterations in early carcinogenesis.
Quantification of nanoscale density fluctuations by electron microscopy: probing cellular alterations in early carcinogenesis.
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DOI：
10.1088/1478-3975/8/2/026012
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发表时间：
2011-04
期刊：
影响因子：
2
通讯作者：
Backman V
中科院分区：
文献类型：
--
作者：
Pradhan P;Damania D;Joshi HM;Turzhitsky V;Subramanian H;Roy HK;Taflove A;Dravid VP;Backman V
大多数癌症如果在早期得到诊断和治疗是可以治愈的。最近的研究表明，纳米结构的变化发生在早期癌变过程中的细胞内，这种变化之前，显微镜下明显的组织改变。由此可见，全面询问细胞纳米结构的能力（例如，大分子复合物、DNA、RNA、蛋白质和脂质膜）可能对早期癌发生的诊断至关重要。我们提出了一个研究的纳米质量密度波动的生物组织，通过量化其程度的混乱在纳米尺度。利用人体组织的透射电子显微镜（TEM）图像构建相应的有效无序光学晶格。然后，通过对这些光学晶格在纳米尺度上的空间局域本征函数的逆参与比（IPR）的统计分析，研究了纳米尺度无序的性质。我们的研究结果表明，在人类结肠上皮细胞的疾病增加，在受试者窝藏结肠肿瘤的早期阶段。此外，我们的研究结果强烈表明，增加纳米无序与致瘤性的程度。因此，IPR技术提供了一个切实可行的工具，在癌变的最早阶段检测纳米结构的改变。还讨论了该技术用于早期癌症筛查和检测的潜在应用。
Most cancers are curable if they are diagnosed and treated at an early stage. Recent studies suggest that nanoarchitectural changes occur within cells during early carcinogenesis and that such changes precede microscopically evident tissue alterations. It follows that the ability to comprehensively interrogate cell nanoarchitecture (e.g., macromolecular complexes, DNA, RNA, proteins, and lipid membranes) could be critical to the diagnosis of early carcinogenesis. We present a study of the nanoscale mass-density fluctuations of biological tissues by quantifying their degree of disorder at the nanoscale. Transmission electron microscopy (TEM) images of human tissues are used to construct corresponding effective disordered optical lattices. The properties of nanoscale disorder are then studied by statistical analysis of the inverse participation ratio (IPR) of the spatially localized eigenfunctions of these optical lattices at the nanoscale. Our results show an increase in the disorder of human colonic epithelial cells in subjects harboring early stages of colon neoplasia. Furthermore, our findings strongly suggest that increased nanoscale disorder correlates with the degree of tumorigenicity. Therefore, the IPR technique provides a practicable tool for the detection of nanoarchitectural alterations in the earliest stages of carcinogenesis. Potential applications of the technique for early cancer screening and detection are also discussed.
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影响因子：
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通讯作者：
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