Schisandra chinensis reverses visceral hypersensitivity in a neonatal-maternal separated rat model.
Schisandra chinensis reverses visceral hypersensitivity in a neonatal-maternal separated rat model.
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DOI：
10.1016/j.phymed.2011.11.013
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发表时间：
2012-03-15
期刊：
影响因子：
7.9
通讯作者：
Che, Chun-Tao
中科院分区：
文献类型：
--
作者：
Yang, Jia-Ming;Xian, Yan-Fang;Ip, Paul S. P.;Wu, Justin C. Y.;Lao, Lixing;Fonge, Harry H. S.;Sung, Joseph J. Y.;Berman, Brian;Yeung, John H. K.;Che, Chun-Tao
内脏高敏感性是肠易激综合征(IBS)等功能性胃肠疾病的重要特征。本研究在IBS大鼠模型上观察了五味子对新生儿母体分离(NMS)所致内脏痛敏的影响。用腹部收缩反射(AWR)和肌电活动(EMG)检测结肠球囊扩张(CRD)后的内脏运动反应。与正常大鼠相比，接受CRD的NMS对照组大鼠出现了更严重的内脏疼痛，表现为痛阈值降低，AWR评分和肌电反应增强。中药70%乙醇提取物(0.3g/kg和1.5g/kg/d)连续给药7d后，小鼠痛阈值升高(NMS对照组：19.1±1.0 mm Hgvs低剂量：24.8±1.3 mm Hg，高剂量：25.2±1.8 mm Hg，p<；抑制正常大鼠对慢性应激所致的AWR和EMG反应(肌电反应曲线AUC值：正常对照组1952±202vs1074±90，高剂量组1145±92，p<；0.001)，提示羊栖菜可逆转早期应激事件所致的内脏高敏感性。ELSA测定结果表明，经中药治疗后，NMS大鼠远端结肠组织中升高的5-羟色胺(5-羟色胺)水平恢复到正常水平。此外，中药治疗大鼠痛阈值的提高与远端结肠中5-HT3受体的mRNA水平下降有关。以上结果表明，羊栖菜能逆转母婴分离所致的内脏高敏感性，其作用机制可能是通过结肠5-羟色胺途径实现的。
Visceral hypersensitivity is an important characteristic feature of functional gastrointestinal disorders, such as irritable bowel syndrome (IBS). This study evaluated the effect of Schisandra chinensis on visceral hyperalgesia induced by neonatal maternal separation (NMS) in an IBS rat model. The visceromotor responses to colorectal balloon distension (CRD) were measured by abdominal withdrawal reflex (AWR) and electromyographic activities (EMG). NMS control rats (receiving vehicle) underwent aggravated visceral pain in response to CRD as compared to normal rats, evidenced by the reduced pain threshold, enhanced AWR scores and EMG responses. Treatment with a 70% ethanol extract of S. chinensis (0.3 g/kg and 1.5 g/kg per day) for seven days resulted in an increase in the pain threshold (NMS control: 19.1 ± 1.0 mmHg vs low-dose: 24.8 ± 1.3 mmHg and high-dose: 25.2 ± 1.8 mmHg, p<0.01), and abolished the elevated AWR and EMG responses to CRD in NMS rats (AUC values of EMG response curve were: 1952 ± 202 in NMS control group vs 1074 ± 90 in low-dose group and 1145 ± 92 in high-dose group, p<0.001), indicating that S. chinensis could reverse the visceral hypersensitivity induced by early-life stress event. The result of ELSA measurement shows that the elevated serotonin (5-HT) level in the distal colon of NMS rats returned to normal level after treatment with S. chinensis. Moreover, the increase in pain threshold in rats treated with S. chinensis was associated with a decline of the mRNA level of 5-HT3 receptor in the distal colon. All available results demonstrate that S. chinensis can reverse visceral hypersensitivity induced by neonatal-maternal separation, and the effect may be mediated through colonic 5-HT pathway in the rat.
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