Long-term in vitro culture of grape berries and its application to assess the effects of sugar supply on anthocyanin accumulation.
Long-term in vitro culture of grape berries and its application to assess the effects of sugar supply on anthocyanin accumulation.
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DOI：
10.1093/jxb/ert489
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发表时间：
2014-08
影响因子：
6.9
通讯作者：
Gomès E
中科院分区：
文献类型：
--
作者：
Dai ZW;Meddar M;Renaud C;Merlin I;Hilbert G;Delrot S;Gomès E
建立了完整葡萄果实的长期离体培养体系，通过糖对花青苷积累的调节，研究了果实成分对各种营养因子的响应。葡萄果实的发育和成熟受到营养物质、激素和环境信号的复杂调控。然而，这些类型的调节的生化和分子机制知之甚少。一个简化的，但现实的模型系统，使水果生长条件容易调制将有助于破译这些机制。本研究将温室果插生产与离体器官培养技术相结合，建立了葡萄离体浆果离体培养体系。13 C和15 N标记实验表明，该系统使完整的离体浆果能够积极地吸收和利用培养基中的碳和氮。进一步研究了糖对花色素苷积累的影响。在不添加外源脱落酸的情况下，蔗糖浓度>2%时可诱导花色素苷的合成。蔗糖浓度越高，诱导花青苷积累的时间越早。葡萄糖、果糖和蔗糖增加花色素苷的积累，葡萄糖和果糖比蔗糖更有效。这种增加不是由于其前体水平的增加，因为高糖供应降低了苯丙氨酸含量。相反，全基因组转录组分析表明，糖诱导的花青素积累的增强是由于调节和结构基因（特别是UDP-葡萄糖：花青素3-O-葡萄糖基转移酶）的表达改变，以及信号转导途径中的大规模重编程。该体外系统可用于研究浆果成分对营养因子和激素的响应，以及它们与环境因子（例如光和温度）的相互作用，这些都可以被精细地调节和控制。
A long-term in vitro culture system of intact grape berries was developed which can serve to study the response of berry composition to various trophic factors, shown by sugar regulation of anthocyanin accumulation. Grape berry development and ripening are under complex regulation by the nutrients, hormones, and environment cues sensed by the berry. However, the biochemical and molecular mechanisms underlying these types of regulation are poorly understood. A simplified but realistic model system that enables fruit growth conditions to be modulated easily will facilitate the deciphering of these mechanisms. Here, an in vitro culture system of intact detached grape berries was developed by coupling the production of greenhouse fruiting-cuttings and in vitro organ culture techniques. 13C and 15N labelling experiments showed that this system enables the intact detached berries actively to absorb and utilize carbon and nitrogen from the culture medium. It was further used to study the effects of sugars on anthocyanin accumulation. A sucrose concentration >2% could induce anthocyanin synthesis in the absence of additional exogenous abscisic acid. The higher the sucrose concentration, the earlier was the induction of anthocyanin accumulation. Glucose, fructose, and sucrose increased anthocyanin accumulation, with glucose and fructose being more effective than sucrose. This increase was not due to an increase in its precursor level, since the phenylalanine content was decreased by a high sugar supply. Instead, genome-wide transcriptome analysis suggests that the sugar-induced enhancement of anthocyanin accumulation results from altered expression of regulatory and structural genes (especially UDP-glucose:anthocyanidin 3-O-glucosyltransferase), together with massive reprogramming in signalling transduction pathways. This in vitro system may serve to study the response of berry composition to nutrient factors and hormones, and their interaction with environmental factors (e.g. light and temperature), which can all be finely tuned and controlled.
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影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1139/b90-236
发表时间：
1990-08-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
CORMIER, F;CREVIER, HA;DO, CB
通讯作者：
DO, CB
影响因子：
4.3
作者：
CANDOLFIVASCONCELOS, MC;CANDOLFI, MP;KOBLET, W
通讯作者：
KOBLET, W
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y