Inhibition of mouse fertilization in vivo by intra-oviductal injection of an anti-equatorin monoclonal antibody.
Inhibition of mouse fertilization in vivo by intra-oviductal injection of an anti-equatorin monoclonal antibody.
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通过输卵管内注射抗赤道蛋白单克隆抗体抑制小鼠体内受精。
DOI：
10.1530/rep.0.1220649
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发表时间：
2001
期刊：
影响因子：
3.8
通讯作者：
K. Toshimori
中科院分区：
文献类型：
--
作者：
K. Yoshinaga;D. Saxena;T. Oh;I. Tanii;K. Toshimori
单克隆抗体 mMN9 可识别抗原分子赤道素，该分子位于哺乳动物精子顶体的赤道段。我们之前使用 IVF 系统的结果表明 mMN9 阻止了精子与卵母细胞的融合。将含有抗体的溶液和对照溶液分别直接注射到麻醉雌性小鼠的右侧和左侧输卵管壶腹中，以评估 mMN9 对体内受精的影响。经过 hCG 处理后，雌性进行交配，并检查其输卵管卵和植入的胚胎。注射后20小时，mMN9保留在输卵管腔内。受精率和妊娠率均显着低于对照组（P < 0.05）。此外，组织学研究表明，注射后没有证据表明女性生殖道发生病理变化。这些结果表明mMN9在体内条件下显着抑制小鼠受精，并且这种注射方法对于研究抗体和药剂对体内受精的影响应该是有用的。
The monoclonal antibody mMN9 recognizes an antigenic molecule, equatorin, which is localized at the equatorial segment of the mammalian sperm acrosome. Our previous results using an IVF system indicated that mMN9 blocked sperm-oocyte fusion. Antibody-containing and control solutions were injected directly into the right and left oviductal ampullae, respectively, of anaesthetized female mice to assess the effect of mMN9 on fertilization in vivo. After hCG treatment, the females were mated, and their oviductal eggs and implanted embryos were examined. mMN9 was retained in the oviductal lumen at 20 h after injection. The rates of fertilization and concomitant pregnancy were significantly lower than in the control side (P < 0.05). In addition, histological studies showed no evidence of pathological changes in the female reproductive tract after the injections. These results indicate that mMN9 inhibits mouse fertilization significantly under in vivo conditions and that this injection method should be useful for studying the effects of antibodies and agents on fertilization in vivo.
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使用源自配子（精子和透明带）和激素（人绒毛膜促性腺激素）的抗原开发人类避孕疫苗：现状。
影响因子：
13.3
作者：
Naz,RK;Sacco,A;Singh,O;Pal,R;Talwar,GP
通讯作者：
Talwar,GP
DOI：
10.2741/evans
发表时间：
1999-01-15
期刊：
Frontiers in Bioscience
影响因子：
--
作者：
Evans, Janice P.
通讯作者：
Evans, Janice P.
影响因子：
3.6
作者：
Saling,PM;Waibel,R
通讯作者：
Waibel,R
影响因子：
2.7
作者：
Saling,PM
通讯作者：
Saling,PM