Moderate Maternal Energy Restriction During Gestation in Pigs Attenuates Fetal Skeletal Muscle Development Through Changing Myogenic Gene Expression and Myofiber Characteristics
Moderate Maternal Energy Restriction During Gestation in Pigs Attenuates Fetal Skeletal Muscle Development Through Changing Myogenic Gene Expression and Myofiber Characteristics
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猪妊娠期间适度的母体能量限制会通过改变生肌基因表达和肌纤维特征来减弱胎儿骨骼肌的发育
DOI：
10.1177/1933719116651151
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发表时间：
2017-01
影响因子：
2.9
通讯作者：
Chen Daiwen
中科院分区：
文献类型：
--
作者：
Zou Ti;e;He Dongting;Yu Bing;Yu Jie;Mao Xiangbing;Zheng Ping;He Jun;Huang Zhiqing;Chen Daiwen
母亲在怀孕期间营养不良会影响胎儿的发育，从而增加成年后患代谢综合征的风险。骨骼肌是关键的胰岛素反应器官之一，易受母体营养计划的影响。本研究旨在评价猪妊娠期母体适度能量限制对胎儿骨骼肌发生基本事件的影响。从交配到妊娠90天，对初产纯种梅山母猪饲喂对照（正常能量摄入）或低能量（LE）日粮。在妊娠第55天和第90天，对胎儿背最长肌（LD）的生化特征、肌源性基因表达和肌纤维特征进行了评估。LE组在妊娠第55天和第90天的胎儿体重、肌纤维密度和胎儿脐静脉血清三碘甲状腺原氨酸（T3）浓度均降低。在妊娠55或90天的LE胎儿的LD肌肉中，肌酸激酶的表达和活性、肌球蛋白重链（MYH/MyHC）基因（MYH 2和MYH 4）的信使RNA（mRNA）表达、肌肉DNA和蛋白质浓度以及快速-MyHC异构体的蛋白质表达降低。同时，在妊娠第55或90天LE胎儿中，肌生成基因表达减少，表明肌生成下调。此外，暴露于LE饮食导致增加异柠檬酸脱氢酶活性和缓慢的MyHC（MYH 7）mRNA表达在妊娠90天，这表明氧化肌肉代谢的升高。这些结果表明，在妊娠期适度的能量限制衰减胎儿骨骼肌的发育，导致骨骼肌分化和成熟的延迟。
Maternal undernutrition during gestation influences the development of the fetus, thereby increasing the risk of metabolic syndrome in adulthood. Skeletal muscle, one of the key insulin-responsive organs, is susceptible to maternal nutritional programming. This study aimed to evaluate the effect of moderate maternal energy restriction during gestation in pigs on basic events of fetal skeletal myogenesis. Primiparous, purebred Meishan sows were fed a control (normal energy intake) or a low-energy (LE) diet from mating to day 90 of gestation. Biochemical characteristics, myogenic gene expression, and myofiber characteristics were assessed in longissimus dorsi (LD) muscle of fetuses on days 55 and 90 of gestation. Fetal weights, myofiber density, and fetal umbilical vein serum triiodothyronine (T3) concentration decreased in LE group on both days 55 and 90 of gestation. The expression and activity of creatine kinase, the messenger RNA (mRNA) expression of myosin heavy chain (MYH/MyHC) genes (MYH2 and MYH4), concentration of muscular DNA and protein, and protein expression of fast-MyHC isoforms were reduced in LD muscle of LE fetuses on day 55 or 90 of gestation. Meanwhile, myogenic gene expression was reduced in LE fetuses on day 55 or 90 of gestation, indicating the downregulation of myogenesis. Additionally, the exposure to LE diet led to increased isocitrate dehydrogenase activity and slow MyHC (MYH7) mRNA expression on day 90 of gestation, suggesting the elevation of oxidative muscle metabolism. These findings suggest that moderate energy restriction during gestation attenuates fetal skeletal muscle development in pigs, resulting in the delay of skeletal muscle differentiation and maturity.
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影响因子：
3.3
作者：
G. Foxcroft;W. Dixon;S. Novak;C. T. Putman;S. Town;M. Vinsky
通讯作者：
G. Foxcroft;W. Dixon;S. Novak;C. T. Putman;S. Town;M. Vinsky
影响因子：
5.3
作者：
Jane E. Johnson;B. Wold;S. Hauschka
通讯作者：
Jane E. Johnson;B. Wold;S. Hauschka
影响因子：
2.4
作者：
P. Wigmore;N. Stickland
通讯作者：
P. Wigmore;N. Stickland
适度增加母体膳食能量摄入会延迟猪胎儿骨骼肌分化和成熟
影响因子：
5
作者：
Zou Ti;e;He Dongting;Yu Bing;Yu Jie;Mao Xiangbing;Zheng Ping;He Jun;Huang Zhiqing;Shu Yan;Liu Yue;Chen Daiwen
通讯作者：
Chen Daiwen
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