Halioticida noduliformans infection in eggs of lobster (Homarus gammarus) reveals its generalist parasitic strategy in marine invertebrates.
Halioticida noduliformans infection in eggs of lobster (Homarus gammarus) reveals its generalist parasitic strategy in marine invertebrates.
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DOI：
10.1016/j.jip.2018.03.002
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发表时间：
2018-05
影响因子：
3.4
通讯作者：
Bass D
中科院分区：
文献类型：
--
作者：
Holt C;Foster R;Daniels CL;van der Giezen M;Feist SW;Stentiford GD;Bass D
在欧洲龙虾卵和鳃中首次记录到结节皱纹盘藻。在欧洲龙虾蛋中检测到与卡氏拉格尼迪斯杆菌的混合感染。系统发育分析证实‘Haliphthoros sp.NJM 0034‘为结瘤链球菌属。新的卵菌特异性18S rRNA基因聚合酶链式反应引物的开发和测试。卵菌特异的引物可以检测环境样品中的广泛多样性。从欧洲龙虾(Homarus Gammarus)变色的卵中分离到一种具有卵菌样形态和致病作用的寄生虫，后来在成年龙虾的鳃组织中发现了这种寄生虫。设计了群特异性卵菌引物来扩增18S核糖体小亚基(SSU)，初步鉴定该菌与Haliphthoros sp.相同。NJM 0034菌株(AB178865.1)，以前从鲍鱼(从南澳大利亚进口到日本)中分离出来。然而，根据其他已发表的基于SSU的系统发育树，NJM 0034分离物在我们的最大似然和贝叶斯系统发育图中没有与其他已知的Haliphthoros物种聚在一起。相反，该菌株形成了一个孤儿谱系，在Saprolegniales和Pythiales分离之前出现了分歧。根据28S大亚基(LSU)系统发育树，我们自己的分离物和之前未鉴定的0034菌株都与鲍鱼病原菌Halitisida nuuliformans完全相同。该属在形态上与Haliphthoros和Halcrustiida相似，并在LSU的系统发育中与它们形成一个支系。在这里，我们确认了在欧洲龙虾中首次记录到结节杆菌的存在，并将其存在与卵块的病理联系起来，这可能导致生殖力下降。
First record of Halioticida noduliformans in European lobster eggs and gills. Coinfection with Lagenidium callinectes detected in European lobster eggs. Phylogenetic analyses confirm ‘Haliphthoros sp. NJM 0034’ is H. noduliformans. Novel Oomycete-specific 18S rRNA gene PCR primers developed and tested. Oomycete-specific primers detect wide diversity in environmental samples. A parasite exhibiting Oomycete-like morphology and pathogenesis was isolated from discoloured eggs of the European lobster (Homarus gammarus) and later found in gill tissues of adults. Group-specific Oomycete primers were designed to amplify the 18S ribosomal small subunit (SSU), which initially identified the organism as the same as the ‘Haliphthoros’ sp. NJM 0034 strain (AB178865.1) previously isolated from abalone (imported from South Australia to Japan). However, in accordance with other published SSU-based phylogenies, the NJM 0034 isolate did not group with other known Haliphthoros species in our Maximum Likelihood and Bayesian phylogenies. Instead, the strain formed an orphan lineage, diverging before the separation of the Saprolegniales and Pythiales. Based upon 28S large subunit (LSU) phylogeny, our own isolate and the previously unidentified 0034 strain are both identical to the abalone pathogen Halioticida noduliformans. The genus shares morphological similarities with Haliphthoros and Halocrusticida and forms a clade with these in LSU phylogenies. Here, we confirm the first recorded occurrence of H. noduliformans in European lobsters and associate its presence with pathology of the egg mass, likely leading to reduced fecundity.
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影响因子：
1.4
作者：
Chukanhom, Kanit;Borisutpeth, Prasatporn;Hatai, Kishio
通讯作者：
Hatai, Kishio
影响因子：
1.4
作者：
Kitancharoen, Nilubol;Hatai, Kishio
通讯作者：
Hatai, Kishio
影响因子：
2.8
作者：
BLAND, CE;AMERSON, HV
通讯作者：
AMERSON, HV
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
--
作者：
DIEGUEZURIBEONDO, J;CERENIUS, L;SODERHALL, K
通讯作者：
SODERHALL, K