Antimicrobial Particulate Silver Coatings on Stainless Steel Implants for Fracture Management.
Antimicrobial Particulate Silver Coatings on Stainless Steel Implants for Fracture Management.
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DOI：
10.1016/j.msec.2012.02.020
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发表时间：
2012-07-01
影响因子：
7.9
通讯作者：
Zirkle, Lewis G.
中科院分区：
文献类型：
--
作者：
DeVasConCellos, Paul;Bose, Susmita;Beyenal, Haluk;Bandyopadhyay, Amit;Zirkle, Lewis G.
我们已经在不锈钢上使用微粒银涂层来防止体内细菌感染。不锈钢通常用作骨折治疗的植入物材料。银的抗菌用途已得到充分记录和研究，因此本研究的新奇在于使用颗粒涂层以及面对骨折修复植入物的真实的世界挑战。用于施加涂层的可变参数是沉积时间、银溶液浓度、施加的电压、400至500 ℃之间的热处理温度和时间。使用MTT增殖测定和SEM形态图像显示所得涂层对人成骨细胞无毒。使用模拟体液进行各种处理的体外银释放研究。通过在生物反应器中用铜绿假单胞菌挑战涂层来测试杀菌效果，并与未涂覆的不锈钢进行比较。在24小时时观察到细菌减少13倍，并证明具有统计学显著性。
We have used particulate silver coating on stainless steel to prevent in vivo bacterial infection. Stainless steel is commonly used as an implant material for fracture management. The antimicrobial use of silver has been well documented and studied, therefore the novelty of this research is the use of a particulate coating as well as facing the real world challenges of a fracture repair implant. The variable parameters for applying the coating were time of deposition, silver solution concentration, voltage applied, heat treatment temperature between 400 to 500 °C and time. The resultant coating is shown to be non-toxic to human osteoblasts using an MTT assay for proliferation and SEM images for morphology. In vitro silver release studies of various treatments were done using simulated body fluid. The bactericidal effects were tested by challenging the coatings with P. aeruginosa in a bioreactor and compared against uncoated stainless steel. A 13-fold reduction in bacteria was observed at 24 hours and proved to be statistically significant.
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影响因子：
9.7
作者：
Das, Kakoli;Bose, Susmita;Bandyopadhyay, Amit
通讯作者：
Bandyopadhyay, Amit
影响因子：
14
作者：
Balazs, DJ;Triandafillu, K;Mathieu, HJ
通讯作者：
Mathieu, HJ
影响因子：
3.5
作者：
Esteban-Tejeda, L.;Malpartida, F.;Moya, J. S.
通讯作者：
Moya, J. S.
DOI：
10.1007/bf01142556
发表时间：
1989-01-01
期刊：
Biology of metals
影响因子：
--
作者：
Gadd, G M;Laurence, O S;Trevors, J T
通讯作者：
Trevors, J T
影响因子：
--
作者：
Hallab, NJ;Bundy, KJ;Jacobs, JJ
通讯作者：
Jacobs, JJ