Collagen denaturation is initiated upon tissue yield in both positional and energy-storing tendons.
Collagen denaturation is initiated upon tissue yield in both positional and energy-storing tendons.
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DOI：
10.1016/j.actbio.2020.09.056
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发表时间：
2020-12
影响因子：
9.7
通讯作者：
Weiss JA
中科院分区：
文献类型：
--
作者：
Lin AH;Allan AN;Zitnay JL;Kessler JL;Yu SM;Weiss JA
肌腱是在肌肉和骨骼之间传递负荷的胶原质软组织。根据其解剖功能，肌腱被分类为具有不同生物力学和生物化学特性的位置或能量储存。我们最近证明，在单调拉伸的位置肌腱，永久变性胶原蛋白开始积累后，离开线性区域的应力-应变曲线。然而，这是未知的，如果这一观察是真实的，在机械过载的其他类型的肌腱。因此，本研究的目的是研究相对于施加的应变的胶原蛋白变性的开始，以及两种肌腱类型之间是否存在差异。大鼠尾腱（RTT）束和大鼠趾长屈肌腱（FDL）的位置和储能肌腱，分别。将样品拉伸至递增应变水平，然后用荧光标记的胶原杂交肽（CHP）染色;测量CHP荧光以定量变性胶原。当样品开始永久变形时，在离开应力-应变曲线的线性区域后，在屈服应变下，位置肌腱和储能肌腱中的变性胶原蛋白开始增加。尽管两种肌腱类型之间存在显著差异，但似乎胶原蛋白变性在单调拉伸期间的组织屈服时开始，并且失效的基本机制是相同的。在组织失效时，定位肌腱的变性胶原蛋白百分比是储能肌腱的两倍，0%对照组之间没有差异。这些结果对理解急性和慢性肌腱损伤都有意义，这将有望改善治疗效果。
Tendons are collagenous soft tissues that transmit loads between muscles and bones. Depending on their anatomical function, tendons are classified as positional or energy-storing with differing biomechanical and biochemical properties. We recently demonstrated that during monotonic stretch of positional tendons, permanent denatured collagen begins accumulating upon departing the linear region of the stress-strain curve. However, it is unknown if this observation is true during mechanical overload of other types of tendons. Therefore, the purpose of this study was to investigate the onset of collagen denaturation relative to applied strain, and whether it differs between the two tendon types. Rat tail tendon (RTT) fascicles and rat flexor digitorum longus (FDL) tendons represented positional and energy-storing tendons, respectively. The samples were stretched to incremental levels of strain, then stained with fluorescently labeled collagen hybridizing peptides (CHPs); the CHP fluorescence was measured to quantify denatured collagen. Denatured collagen in both positional and energy-storing tendons begins to increase at the yield strain, upon leaving the linear region of the stress-strain curve as the sample begins to permanently deform. Despite significant differences between the two tendon types, it appears that collagen denaturation is initiated at tissue yield during monotonic stretch, and the fundamental mechanism of failure is the same. At tissue failure, positional tendons had double the percentage of denatured collagen compared to energy-storing tendons, with no difference between 0% control groups. These results have implications in understanding both acute and chronic tendon injuries, which will hopefully lead to improved treatment outcomes.
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DOI：
10.1002/jor.20722
发表时间：
2009-02
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Fung DT;Wang VM;Laudier DM;Shine JH;Basta-Pljakic J;Jepsen KJ;Schaffler MB;Flatow EL
通讯作者：
Flatow EL
影响因子：
4.6
作者：
Quigley AS;Bancelin S;Deska-Gauthier D;Légaré F;Kreplak L;Veres SP
通讯作者：
Veres SP
影响因子：
3.8
作者：
Elliott, DM;Robinson, PS;Soslowsky, LJ
通讯作者：
Soslowsky, LJ
影响因子：
9.7
作者：
Herod, Tyler W.;Chambers, Neil C.;Veres, Samuel P.
通讯作者：
Veres, Samuel P.
影响因子：
3.8
作者：
Duenwald-Kuehl, Sarah;Kondratko, Jaclyn;Vanderby, Ray, Jr.
通讯作者：
Vanderby, Ray, Jr.