Synthesis of multifunctional cellulose nanocrystals for lectin recognition and bacterial imaging.
Synthesis of multifunctional cellulose nanocrystals for lectin recognition and bacterial imaging.
复制标题
DOI：
10.1021/acs.biomac.5b00227
复制
发表时间：
2015-04-13
影响因子：
6.2
通讯作者：
Ramstrom, Olof
中科院分区：
文献类型：
--
作者：
Zhou, Juan;Butchosa, Nuria;Jayawardena, H. Surangi N.;Park, JaeHyeung;Zhou, Qi;Yan, Mingdi;Ramstrom, Olof
多功能纤维素纳米晶作为一种新型的糖纳米材料在凝集素结合和细菌成像中得到了广泛的应用。通过TEMPO介导的氧化和酸水解，然后用喹诺酮荧光团和碳水化合物配体官能化制备纤维素纳米晶体。纤维素纳米晶体随后被应用于与碳水化合物结合蛋白的相互作用研究和细菌成像。结果表明，功能化纤维素纳米晶可以选择性识别相应的同源凝集素。此外，甘露糖基化的纳米晶体显示出选择性地与FimH呈递的E.用透射电镜和共聚焦荧光显微镜观察到。这些糖纳米材料为纤维素纳米晶体在生物识别和成像方面提供了新的应用。
Multifunctional cellulose nanocrystals have been synthesized and applied as a new type of glyconanomaterial in lectin binding and bacterial imaging. The cellulose nanocrystals were prepared by TEMPO-mediated oxidation and acidic hydrolysis, followed by functionalization with a quinolone fluorophore and carbohydrate ligands. The cellulose nanocrystals were subsequently applied in interaction studies with carbohydrate-binding proteins and in bacterial imaging. The results show that the functional cellulose nanocrystals could selectively recognize the corresponding cognate lectins. In addition, mannosylated nanocrystals were shown to selectively interact with FimH-presenting E. coli, as detected by TEM and confocal fluorescence microscopy. These glyconanomaterials provide a new application of cellulose nanocrystals in biorecognition and imaging.
登录
查看更多内容
影响因子：
9.9
作者：
Chen X;Ramström O;Yan M
通讯作者：
Yan M
影响因子：
4.3
作者：
Kennedy, David C.;Gruenstein, Dan;Seeberger, Peter H.
通讯作者：
Seeberger, Peter H.
影响因子：
3
作者：
LAPICQUE, F;DELLACHERIE, E
通讯作者：
DELLACHERIE, E
影响因子：
6.2
作者：
Clift, Martin J. D.;Foster, E. Johan;Weder, Christoph
通讯作者：
Weder, Christoph
DOI：
10.1039/c3cc40491a
发表时间：
2013-04-14
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Jayawardena HS;Jayawardana KW;Chen X;Yan M
通讯作者：
Yan M