The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, CV 777
The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, CV 777
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DOI：
10.1016/0378-1135(81)90007-9
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发表时间：
1981-06
影响因子：
3.3
通讯作者：
Coussement W
中科院分区：
文献类型：
--
作者：
Debouck P;Pensaert M;Coussement W
16头2-3日龄的剖腹产、断乳仔猪，每头经口鼻腔给药2毫升的无菌滤液，含104头猪感染的CV777。感染后每隔12~120h处死仔猪。冠状病毒样病原体引起肠道局部感染，导致绒毛萎缩、吸收不良和腹泻。这种感染的发病机制与猪传染性胃肠炎(TGE)相似，TGE是一种已知的猪冠状病毒感染。然而，在哪里有一些不同。免疫荧光染色显示，CV777抗原不仅存在于覆盖小肠绒毛的上皮细胞中，也存在于结肠表面上皮细胞中。小肠隐窝上皮偶见荧光，但隐窝的再生能力未受影响。CV777感染肠上皮细胞的进展明显慢于TGE，潜伏期较长，对肠上皮细胞的破坏较轻。在CV777感染的后期，再生细胞的感染程度比TGE中观察到的要高得多。
Sixteen 2–3-days-old caesarean-derived, colostrum-deprived piglets were each dosed oro-nasally with 2 ml of a bacteria-free filtrate containing 104 pig-infectious-doses of CV 777. The piglets were killed at intervals of 12 to 120 h after infection. The coronvirus-like agent caused a local infection of the intestinal tract which resulted in villous atrophy, malabsorption and diarrhea. The pathogenesis of this infection was similar to that of transmissible gastroenteritis (TGE), a known coronaviral infection of pigs. However, where were some differences. By immunofluorescent staining, CV 777 antigens were not only detected in the epithelial cells covering the small intestinal villi, but also in the cells of the colonic surface epithelium. Occasional fluorescence was also seen in the small intestinal crypt epithelium, but the regenerative capacity of the crypts was not affected. The progress of intestinal epithelial cell infection by CV 777 was much slower than that in TGE, resulting in a longer incubation period and in less drastic epithelial cell destruction. The infection of regenerating cells occurred to a much higher degree during the late stage of a CV 777 infection than has been observed in TGE.
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影响因子：
0.8
作者：
PEARSON, GR;MCNULTY, MS
通讯作者：
MCNULTY, MS
影响因子：
15.9
作者：
BUTLER, DG;GALL, DG;HAMILTON, JR
通讯作者：
HAMILTON, JR
DOI：
10.1007/bf01253767
发表时间：
1970-01-01
期刊：
ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG
影响因子：
--
作者：
PENSAERT, M;HAELTERMAN, EO;BURNSTEIN, T
通讯作者：
BURNSTEIN, T
DOI：
10.1007/bf01253768
发表时间：
1970-01-01
期刊：
ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG
影响因子：
--
作者：
PENSAERT, M
通讯作者：
PENSAERT, M
影响因子：
2.4
作者：
MEBUS, CA;STAIR, EL;TWIEHAUS, MJ
通讯作者：
TWIEHAUS, MJ