An electrochemiluminescence biosensor for mercury ion detection based on gamma-polyglutamic acid-graphene-luminol composite and oligonucleotides
An electrochemiluminescence biosensor for mercury ion detection based on gamma-polyglutamic acid-graphene-luminol composite and oligonucleotides
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基于γ-聚谷氨酸-石墨烯-鲁米诺复合物和寡核苷酸的电化学发光汞离子检测生物传感器
DOI：
10.1016/j.snb.2014.12.028
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Xiaohua Jiang
中科院分区：
文献类型：
--
作者：
Zhiyong Guo;Beibei Chen;Zebo Wang;Xiaohua Jiang
开发了一种基于γ-聚谷氨酸-石墨烯-鲁米诺（γ-PGA-G-鲁米诺）复合物和寡核苷酸的超灵敏电化学发光（ECL）生物传感器，用于检测汞离子（Hg2+）。涂覆在玻碳电极上的γ-PGA-G-鲁米诺复合材料可以通过鲁米诺产生强而稳定的ECL信号，并通过γ-PGA提供羧基与富含胸腺嘧啶（T）的氨基修饰的DNA1交联。添加 Hg2+ 和富含 T 的生物素修饰的 DNA2 后，T-Hg2+-T 相互作用发生并介导 DNA1 和 DNA2 之间的协调。随后，通过链霉亲和素与生物素的特异性结合，将链霉亲和素连接到DNA2末端，由于电子传递能力较差的惰性蛋白层，明显降低了ECL强度。基于特异性T-Hg2+-T配位化学和链霉亲和素-生物素系统的特异性结合，获得了超高灵敏度和选择性的Hg2+检测。结果表明，ECL 强度与 Hg2+ 浓度在 0.01 至 100 nmol/L（约 0.002–20 ppb）的较宽范围内呈对数线性关系。此外，该生物传感器还对 Hg2+ 离子表现出优异的选择性，而不受样品基质中常见共存金属离子的显着干扰。出色的灵敏度和选择性使所开发的生物传感器成为检测 Hg2+ 的潜在且简单的工具。
An ultrasensitive electrochemiluminescence (ECL) biosensor based on gamma-polyglutamic acid-graphene-luminol (γ-PGA-G-luminol) composite and oligonucleotides for the detection of mercury ion (Hg2+) was developed. γ-PGA-G-luminol composite coated on the glassy carbon electrode could produce a strong and stable ECL signal by luminol, and provide carboxyl groups to cross-link with amino-modified DNA1 enriching in thymine (T) by γ-PGA. Upon addition of Hg2+and biotin-modified DNA2 enriching in T, T–Hg2+–T interaction occurred and mediated the coordination between DNA1 and DNA2. Afterwards, streptavidin was connected to the end of DNA2 through specific binding of streptavidin to biotin, which obviously reduced the ECL intensity due to the inert protein layer with poor electron transfer ability. Based on the specific T–Hg2+–T coordination chemistry and the specific binding of streptavidin-biotin system, ultrahigh sensitivity and selectivity for Hg2+detection were obtained. Results revealed that the ECL intensity was logarithmically linear with the concentration of Hg2+in a wide range from 0.01 to 100 nmol/L (about 0.002–20 ppb). Moreover, the biosensor also exhibited excellent selectivity for Hg2+ions without significant interference from commonly co-existing metal ions in the sample matrix. Excellent sensitivity and selectivity make the developed biosensor a potential and simple tool for the detection of Hg2+.
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影响因子：
8.9
作者：
Qianrui Wang;Daekeun Kim;D. Dionysiou;G. Sorial;D. Timberlake
通讯作者：
Qianrui Wang;Daekeun Kim;D. Dionysiou;G. Sorial;D. Timberlake
影响因子：
3
作者：
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通讯作者：
Jun Wan;G. Yin;Xiuju Ma;Lingbao Xing;Xiliang Luo
影响因子：
6.7
作者：
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通讯作者：
K. Awsiuk;J. Rysz;P. Petrou;A. Budkowski;A. Bernasik;S. Kakabakos;M. Marzec;I. Raptis
影响因子：
6.6
作者：
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通讯作者：
Zhiyong Guo;Shuping Du;Beibei Chen;Yuhong Sha;Bin Qiu;Xiaohua Jiang;Sui Wang;Xing Li
影响因子：
64.8
作者：
Stankovich, Sasha;Dikin, Dmitriy A.;Ruoff, Rodney S.
通讯作者：
Ruoff, Rodney S.