Tacrolimus reduces scar formation and promotes sciatic nerve regeneration.
Tacrolimus reduces scar formation and promotes sciatic nerve regeneration.
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DOI：
10.3969/j.issn.1673-5374.2012.32.003
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发表时间：
2012-11-15
影响因子：
6.1
通讯作者：
Cao X
中科院分区：
文献类型：
--
作者：
Que J;Cao Q;Sui T;Du S;Zhang A;Kong D;Cao X
在SD大鼠坐骨大孔内切断闭孔内肌腱，用尼龙线缝合，建立坐骨神经切断与修复模型。模型用他克莫司灌胃（4 mg/kg/d）处理0、2、4和6周。在胃内给药6周时收获标本。Masson染色显示，他克莫司治疗后，坐骨神经损伤大鼠神经吻合口的胶原纤维含量和瘢痕面积明显减少。苏木精-伊红染色显示，他克莫司明显增加有髓神经纤维密度、轴突平均直径和髓鞘厚度。他克莫司灌胃给药还导致坐骨神经损伤后腓肠肌湿重和坐骨神经功能指数的恢复率显著增加。上述指标在他克莫司灌胃后6周时改善最为显著。斯皮尔曼等级相关分析显示，神经吻合口有髓神经纤维密度及坐骨神经功能与瘢痕面积呈显著负相关。提示他克莫司可通过减少瘢痕形成促进周围神经再生，加快神经功能恢复。
A sciatic nerve transection and repair model was established in Sprague-Dawley rats by transecting the tendon of obturator internus muscle in the greater sciatic foramen and suturing with nylon sutures. The models were treated with tacrolimus gavage (4 mg/kg per day) for 0, 2, 4 and 6 weeks. Specimens were harvested at 6 weeks of intragastric administration. Masson staining revealed that the collagen fiber content and scar area in the nerve anastomosis of the sciatic nerve injury rats were significantly reduced after tacrolimus administration. Hematoxylin-eosin staining showed that tacrolimus significantly increased myelinated nerve fiber density, average axon diameter and myelin sheath thickness. Intragastric administration of tacrolimus also led to a significant increase in the recovery rate of gastrocnemius muscle wet weight and the sciatic functional index after sciatic nerve injury. The above indices were most significantly improved at 6 weeks after of tacrolimus gavage. The myelinated nerve fiber density in the nerve anastomosis and the sciatic nerve functions had a significant negative correlation with the scar area, as detected by Spearman’s rank correlation analysis. These findings indicate that tacrolimus can promote peripheral nerve regeneration and accelerate the recovery of neurological function through the reduction of scar formation.
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影响因子：
5.3
作者：
DEMEDINACELI, L;CHURCH, AC;WANG, YN
通讯作者：
WANG, YN
影响因子：
3.4
作者：
Navarro, X;Udina, E;Gold, BG
通讯作者：
Gold, BG
影响因子：
5.3
作者：
Costantini, LC;Isacson, O
通讯作者：
Isacson, O
DOI：
10.2106/00004623-196749040-00008
发表时间：
1967-01-01
影响因子：
5.3
作者：
BORA, FW
通讯作者：
BORA, FW
DOI：
10.1080/10731190802375810
发表时间：
2008-01-01
期刊：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
影响因子：
--
作者：
Han Haisheng;Zuo Songjie;Li Xin
通讯作者：
Li Xin