A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
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DOI：
10.1371/journal.pone.0038718
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Cao G;Liu Y;Zhang S;Yang X;Chen R;Zhang Y;Lu W;Liu Y;Wang J;Lin M;Wang G
莽草酸途径中的关键酶5-烯醇丙酮基-3-磷酸合成酶(EPSPS)是广谱除草剂草甘膦的主要靶标。鉴定编码高水平耐草甘膦EPSPS的新的AROA基因对于培育耐草甘膦作物是至关重要的。在本研究中，评估了5个细菌aroA基因在大肠杆菌aroA缺陷菌株ER2799和转基因烟草中对草甘膦的耐受性。所有五个aroA基因都可以补充aroA缺陷菌株ER2799，am79 aroa表现出最高的草甘膦耐受性。虽然草甘膦处理抑制了WT和转基因烟草的生长，但表达am79 AroA的转基因植株比表达其他AroA基因的植株耐受更高的草甘膦浓度，并且有更高的鲜重和成活率。在高浓度草甘膦处理下，转am79 AroA基因植株的叶片中莽草酸含量低于表达其他AroA基因的植株。这些结果表明am79 Aroa可能是开发转基因耐草甘膦作物的一个很好的候选者。
A key enzyme in the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the primary target of the broad-spectrum herbicide glyphosate. Identification of new aroA genes coding for EPSPS with a high level of glyphosate tolerance is essential for the development of glyphosate-tolerant crops. In the present study, the glyphosate tolerance of five bacterial aroA genes was evaluated in the E. coli aroA-defective strain ER2799 and in transgenic tobacco plants. All five aroA genes could complement the aroA-defective strain ER2799, and AM79 aroA showed the highest glyphosate tolerance. Although glyphosate treatment inhibited the growth of both WT and transgenic tobacco plants, transgenic plants expressing AM79 aroA tolerated higher concentration of glyphosate and had a higher fresh weight and survival rate than plants expressing other aroA genes. When treated with high concentration of glyphosate, lower shikimate content was detected in the leaves of transgenic plants expressing AM79 aroA than transgenic plants expressing other aroA genes. These results suggest that AM79 aroA could be a good candidate for the development of transgenic glyphosate-tolerant crops.
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影响因子：
56.9
作者：
COMAI, L;SEN, LC;STALKER, DM
通讯作者：
STALKER, DM
影响因子：
4.8
作者：
Healy-Fried, Martha L.;Funke, Todd;Schoenbrunn, Ernst
通讯作者：
Schoenbrunn, Ernst
影响因子：
2.7
作者：
Liang, Aimin;Sha, Jiying;Lin, Min
通讯作者：
Lin, Min
影响因子：
4.4
作者：
FITZGIBBON, JE;BRAYMER, HD
通讯作者：
BRAYMER, HD
DOI：
10.1038/nbt0687-579
发表时间：
1987-06-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
DELLACIOPPA, G;BAUER, SC;KISHORE, GM
通讯作者：
KISHORE, GM