Functional characterization of calcineurin homologs PsCNA1/PsCNB1 in Puccinia striiformis f. sp. tritici using a host-induced RNAi system.
Functional characterization of calcineurin homologs PsCNA1/PsCNB1 in Puccinia striiformis f. sp. tritici using a host-induced RNAi system.
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条锈菌 f 中钙调神经磷酸酶同系物 PsCNA1/PsCNB1 的功能表征。
DOI：
10.1371/journal.pone.0049262
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kang Z
中科院分区：
文献类型：
--
作者：
Zhang H;Guo J;Voegele RT;Zhang J;Duan Y;Luo H;Kang Z
钙调神经磷酸酶在真菌的形态发生、致病和耐药性中起着关键作用。然而，钙调磷酸酶基因在小麦条锈菌f。sp. triglycerides（Pst）是一种未知的细菌。我们确定和特点的钙调神经磷酸酶基因PsCNA 1和PsCNB 1在Pst。系统发育分析表明，PsCNA 1和PsCNB 1形成钙/钙调素调节的蛋白磷酸酶，属于钙调神经磷酸酶异源二聚体，由亚基A和B组成。定量RT-PCR分析表明，PsCNA 1和PsCNB 1在吸器形成期（接种后1d）表达量最高。利用大麦条纹花叶病毒（BSMV）作为小麦瞬时表达载体，抑制了PsCNA 1和PsCNB 1在Pst中的表达，导致真菌菌丝生长缓慢，夏孢子产量减少。免疫抑制剂环孢菌素A和FK 506显著降低夏孢子的萌发率，当萌发发生时，产生两个以上的芽管。这些结果表明，钙调神经磷酸酶信号通路参与了条锈菌形态发生分化，尤其是在侵染早期和夏孢子产生过程中吸器的形成。因此，PsCNA 1和PsCNB 1可能是参与小麦-Pst互作的重要致病基因。
Calcineurin plays a key role in morphogenesis, pathogenesis and drug resistance in most fungi. However, the function of calcineurin genes in Puccinia striiformis f. sp. tritici (Pst) is unclear. We identified and characterized the calcineurin genes PsCNA1 and PsCNB1 in Pst. Phylogenetic analyses indicate that PsCNA1 and PsCNB1 form a calcium/calmodulin regulated protein phosphatase belonging to the calcineurin heterodimers composed of subunits A and B. Quantitative RT-PCR analyses revealed that both PsCNA1 and PsCNB1 expression reached their maximum in the stage of haustorium formation, which is one day after inoculation. Using barely stripe mosaic virus (BSMV) as a transient expression vector in wheat, the expression of PsCNA1 and PsCNB1 in Pst was suppressed, leading to slower extension of fungal hyphae and reduced production of urediospores. The immune-suppressive drugs cyclosporin A and FK506 markedly reduced the germination rates of urediospores, and when germination did occur, more than two germtubes were produced. These results suggest that the calcineurin signaling pathway participates in stripe rust morphogenetic differentiation, especially the formation of haustoria during the early stage of infection and during the production of urediospores. Therefore PsCNA1 and PsCNB1 can be considered important pathogenicity genes involved in the wheat-Pst interaction.
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影响因子：
3.7
作者：
Guo J;Dai X;Xu JR;Wang Y;Bai P;Liu F;Duan Y;Zhang H;Huang L;Kang Z
通讯作者：
Kang Z
影响因子：
11.4
作者：
Cruz, MC;Fox, DS;Heitman, J
通讯作者：
Heitman, J
影响因子：
7.4
作者：
Burch-Smith, Tessa M.;Schiff, Michael;Dinesh-Kumar, S. P.
通讯作者：
Dinesh-Kumar, S. P.
影响因子：
2.8
作者：
Jinhee, Choi;Kim, Yangseon;Lee, Yong-Hwan
通讯作者：
Lee, Yong-Hwan
番茄果实中病毒诱导的基因沉默。
影响因子：
7.2
作者：
Fu, DQ;Zhu, BZ;Luo, YB
通讯作者：
Luo, YB