Biophysical mechanism of the protective effect of blue honeysuckle (Lonicera caerulea L. var. kamtschatica Sevast.) polyphenols extracts against lipid peroxidation of erythrocyte and lipid membranes.
Biophysical mechanism of the protective effect of blue honeysuckle (Lonicera caerulea L. var. kamtschatica Sevast.) polyphenols extracts against lipid peroxidation of erythrocyte and lipid membranes.
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DOI：
10.1007/s00232-014-9677-5
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发表时间：
2014-07
影响因子：
2.4
通讯作者：
Kleszczynska, H.
中科院分区：
文献类型：
--
作者：
Bonarska-Kujawa, D.;Pruchnik, H.;Cyboran, S.;Zylka, R.;Oszmianski, J.;Kleszczynska, H.
关键词：
本研究的目的是确定蓝金银花果实和叶提取物成分对红细胞和脂质膜的物理性质的影响，并评估其抗氧化性能。高效液相色谱分析表明，提取物中富含果实中的多酚类花青素和叶中的黄酮类化合物。结果表明，这两种提取物具有抗氧化活性，并保护红细胞膜免受紫外线照射和AAPH诱导的氧化。浸提液不诱导溶血，并轻微增加红细胞的渗透阻力。研究表明，提取物成分主要结合在红细胞膜的外部，诱导棘细胞的形成。广义偏振和荧光各向异性的值表明，提取物多酚改变了红细胞和脂质膜的亲水部分的包装安排，而不改变疏水部分的流动性。DSC结果还表明，萃取组分不改变DPPC膜的主要相转变温度。对提取物修饰的膜的电参数的研究表明，它们稍微稳定脂质膜，并且不降低其比电阻或容量。红外光谱检查表明，在提取物的作用下，脂质体亲水区域的水合程度发生了微小变化。多酚化合物位于细胞膜的亲水部分，似乎构成了细胞对其他物质的保护屏障，特别是对氧的反应形式。
The aim of the present research was to determine the effect of blue honeysuckle fruit and leaf extracts components on the physical properties of erythrocyte and lipid membranes and assess their antioxidant properties. The HPLC analysis showed that the extracts are rich in polyphenol anthocyanins in fruits and flavonoids in leaves. The results indicate that both extracts have antioxidant activity and protect the red blood cell membrane against oxidation induced by UVC irradiation and AAPH. The extracts do not induce hemolysis and slightly increase osmotic resistance of erythrocytes. The research showed that extracts components are incorporated mainly in the external part of the erythrocyte membrane, inducing the formation of echinocytes. The values of generalized polarization and fluorescence anisotropy indicate that the extracts polyphenols alter the packing arrangement of the hydrophilic part of the erythrocyte and lipid membranes, without changing the fluidity of the hydrophobic part. The DSC results also show that the extract components do not change the main phase transition temperature of DPPC membrane. Studies of electric parameters of membranes modified by the extracts showed that they slightly stabilize lipid membranes and do not reduce their specific resistance or capacity. Examination of IR spectra indicates small changes in the degree of hydration in the hydrophilic region of liposomes under the action of the extracts. The location of polyphenolic compounds in the hydrophilic part of the membrane seems to constitute a protective shield of the cell against other substances, the reactive forms of oxygen in particular.
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影响因子：
64.8
作者：
MUELLER, P;RUDIN, DO;WESCOTT, WC
通讯作者：
WESCOTT, WC
影响因子：
9.7
作者：
Chen, Pei-Ni;Chu, Shu-Chen;Hsieh, Yih-Shou
通讯作者：
Hsieh, Yih-Shou
影响因子：
3
作者：
Bonarska-Kujawa, Dorota;Pruchnik, Hanna;Oszmianski, Jan;Sarapuk, Janusz;Kleszczynska, Halina
通讯作者：
Kleszczynska, Halina
影响因子：
3.4
作者：
LEWIS, RNAH;MCELHANEY, RN;MANTSCH, HH
通讯作者：
MANTSCH, HH
影响因子：
2.4
作者：
KALINOWSKI, S;FIGASZEWSKI, Z
通讯作者：
FIGASZEWSKI, Z