Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize.
Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize.
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玉米丛枝菌根真菌响应性 microRNA 及其调控网络的鉴定
DOI：
10.3390/ijms19103201
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发表时间：
2018-10-16
影响因子：
5.6
通讯作者：
Cheng B
中科院分区：
文献类型：
--
作者：
Xu Y;Zhu S;Liu F;Wang W;Wang X;Han G;Cheng B
玉米可以与丛枝菌根（AM）真菌形成共生关系以提高产量和抗性，但在玉米中负责这一过程的mirna尚未被发现。在这项研究中，通过对AM真菌定殖的玉米根系的sRNA进行高通量测序，鉴定了155个已知mirna和28个新的mirna。与其他具有am能力的植物相似，大部分鉴定的玉米mirna长度为24nt。接种AM真菌Glomus intraradices后，14个和2个mirna分别显著下调和上调，提示这些mirna在AM共生中可能发挥作用。有趣的是，14个显著下调的已知玉米mirna中有12个属于miR399家族，该家族先前被报道参与了苜蓿和AM真菌之间的相互作用。这一结果表明，miR399家族在不同植物谱系中可能保守地调控AM共生。通路和网络分析表明，这些差异表达的mirna可能通过其靶基因调控玉米在共生过程中的脂质代谢和磷酸盐饥饿反应。miR399家族和miR397家族的几个成员可能参与控制脂肪酸代谢和促进植物向AM真菌的脂质传递。据我们所知，这是关于miRNAs介导脂肪酸从植物到AM真菌的第一篇报道。本研究揭示了mirna在植物与AM真菌共生中的调控作用。
Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing of sRNA in maize roots colonized by AM fungi. Similar to the profiles in other AM-capable plants, a large proportion of identified maize miRNAs were 24 nt in length. Fourteen and two miRNAs were significantly down- and up-regulated in response to AM fungus Glomus intraradices inoculation, respectively, suggesting potential roles of these miRNAs in AM symbiosis. Interestingly, 12 of 14 significantly down-regulated known maize miRNAs belong to the miR399 family, which was previously reported to be involved in the interaction between Medicago truncatula and AM fungi. This result indicated that the miR399 family should regulate AM symbiosis conservatively across different plant lineages. Pathway and network analyses showed that the differentially expressed miRNAs might regulate lipid metabolism and phosphate starvation response in maize during the symbiosis process via their target genes. Several members of the miR399 family and the miR397 family should be involved in controlling the fatty acid metabolism and promoting lipid delivering from plants to AM fungi. To the best of our knowledge, this is the first report on miRNAs mediating fatty acids from plant to AM fungi. This study provides insight into the regulatory roles of miRNAs in the symbiosis between plants and AM fungi.
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影响因子：
5.6
作者：
Fu R;Zhang M;Zhao Y;He X;Ding C;Wang S;Feng Y;Song X;Li P;Wang B
通讯作者：
Wang B
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
16.6
作者：
Jin Y;Liu H;Luo D;Yu N;Dong W;Wang C;Zhang X;Dai H;Yang J;Wang E
通讯作者：
Wang E
影响因子：
30.3
作者：
Couzigou, Jean-Malo;Lauressergues, Dominique;Combier, Jean-Philippe
通讯作者：
Combier, Jean-Philippe
影响因子：
6.4
作者：
Gu, Mian;Xu, Ke;Xu, Guohua
通讯作者：
Xu, Guohua