JAK/STAT and the GATA factor Pannier control hemocyte maturation and differentiation in Drosophila.
JAK/STAT and the GATA factor Pannier control hemocyte maturation and differentiation in Drosophila.
复制标题
DOI：
10.1016/j.ydbio.2011.01.035
复制
发表时间：
2011-04-15
影响因子：
2.7
通讯作者：
Steward R
中科院分区：
文献类型：
--
作者：
Minakhina S;Tan W;Steward R
淋巴腺是果蝇造血的主要场所。在幼虫后期，会产生三种类型的血细胞：浆细胞、晶体细胞和层状细胞，它们的分化受到保守因子和信号通路的严格控制。 JAK/STAT 是在脊椎动物和果蝇造血中具有重要作用的途径之一。我们发现 Stat 在血细胞分化中具有相反的细胞自主和非自主功能。使用克隆方法，我们确定皮质区一组原血细胞中 Stat 的丢失会诱导相邻血细胞中的浆细胞成熟。缺乏 Stat 的血细胞无法分化为浆细胞，表明 Stat 正向且细胞自主地控制浆细胞分化。我们还将 GATA 因子 pannier (pnr) 确定为 Stat 的下游目标。通过分析淋巴腺中 pnr 克隆丢失和过度表达导致的表型，我们发现 Pnr 受 Stat 正向调节，并且是浆细胞分化所必需的。 Stat 和 Pnr 代表控制发育中淋巴腺中血细胞成熟的两个重要因素，并以细胞自主和非细胞自主的方式发挥其功能。
The lymph gland is the major site of hematopoiesis in Drosophila. During late larval stages three types of hemocytes are produced, plasmatocytes, crystal cells, and lamellocytes, and their differentiation is tightly controlled by conserved factors and signaling pathways. JAK/STAT is one of these pathways which have essential roles in vertebrate and fly hematopoiesis. We show that Stat has opposing cell-autonomous and non-autonomous functions in hemocyte differentiation. Using a clonal approach we established that loss of Stat in a set of prohemocytes in the cortical zone induces plasmatocyte maturation in adjacent hemocytes. Hemocytes lacking Stat fail to differentiate into plasmatocytes, indicating that Stat positively and cell-autonomously controls plasmatocyte differentiation. We also identified the GATA factor pannier (pnr) as a downstream target of Stat. By analyzing the phenotypes resulting from clonal loss and over-expression of pnr in lymph glands, we find that Pnr is positively regulated by Stat and specifically required for the differentiation of plasmatocytes. Stat and Pnr represent two essential factors controlling blood cell maturation in the developing lymph gland and exert their functions both in a cell-autonomous and non-cell-autonomous manner.
登录
查看更多内容
影响因子：
1.2
作者：
Bach, Erika A.;Ekas, Laura A.;Baeg, Gyeong-Hun
通讯作者：
Baeg, Gyeong-Hun
影响因子：
10.5
作者：
Harrison, DA;McCoon, PE;Perrimon, N
通讯作者：
Perrimon, N
影响因子：
1.5
作者：
Gajewski, Kathleen M.;Sorrentino, Richard P.;Schulz, Robert A.
通讯作者：
Schulz, Robert A.
影响因子：
2.6
作者：
Fromental-Ramain, Catherine;Vanolst, Luc;Ramain, Philippe
通讯作者：
Ramain, Philippe
影响因子：
8
作者：
Cantor, AB;Orkin, SH
通讯作者：
Orkin, SH