Poised RNA polymerase II changes over developmental time and prepares genes for future expression.
Poised RNA polymerase II changes over developmental time and prepares genes for future expression.
复制标题
DOI：
10.1016/j.celrep.2012.11.024
复制
发表时间：
2012-12-27
期刊：
影响因子：
8.8
通讯作者：
Zeitlinger J
中科院分区：
文献类型：
--
作者：
Gaertner B;Johnston J;Chen K;Wallaschek N;Paulson A;Garruss AS;Gaudenz K;De Kumar B;Krumlauf R;Zeitlinger J
固定的RNA聚合酶II主要是在发育控制基因上发现的，被认为允许其快速和同步诱导细胞外信号。未知在开发过程中如何调节固定RNA POL II的募集。通过在分化的五个阶段将肌肉组织从果蝇胚胎中分离出来，我们表明，在从头开始的许多基因中，有固定的pol II的募集发生，这使得它们允许将来的基因表达。与其他组织相比，这些变化是特异性的，不是组织特异性的。相比之下，多肉体组抑制是组织特异性的，并与Pol II（平衡态）结合使用高度动态表达的基因。这表明固定的pol II受到时间调节，并以组织特异性的方式进行检查。我们将数据与哺乳动物胚胎干细胞进行比较，并讨论一个基于染色质状态的发展程序的框架。
Poised RNA polymerase II is predominantly found at developmental control genes and is thought to allow their rapid and synchronous induction in response to extracellular signals. How the recruitment of poised RNA Pol II is regulated during development is not known. By isolating muscle tissue from Drosophila embryos at five stages of differentiation, we show that the recruitment of poised Pol II occurs at many genes de novo and this makes them permissive for future gene expression. When compared to other tissues, these changes are stage-specific and not tissue-specific. In contrast, Polycomb group repression is tissue-specific and in combination with Pol II (the balanced state) marks genes with highly dynamic expression. This suggests that poised Pol II is temporally regulated and is held in check in a tissue-specific fashion. We compare our data to mammalian embryonic stem cells and discuss a framework for predicting developmental programs based on chromatin state.
登录
查看更多内容
影响因子：
56.9
作者：
Furlong, EEM;Andersen, EC;Scott, MP
通讯作者：
Scott, MP
影响因子：
5.3
作者：
GILMOUR, DS;LIS, JT
通讯作者：
LIS, JT
影响因子：
30.8
作者：
He, Qiye;Bardet, Anais F.;Zeitlinger, Julia
通讯作者：
Zeitlinger, Julia
影响因子：
23.9
作者：
Brookes, Emily;de Santiago, Ines;Hebenstreit, Daniel;Morris, Kelly J.;Carroll, Tom;Xie, Sheila Q.;Stock, Julie K.;Heidemann, Martin;Eick, Dirk;Nozaki, Naohito;Kimura, Hiroshi;Ragoussis, Jiannis;Teichmann, Sarah A.;Pombo, Ana
通讯作者：
Pombo, Ana
影响因子：
64.5
作者：
Guenther, Matthew G.;Levine, Stuart S.;Young, Richard A.
通讯作者：
Young, Richard A.