Monoamines differentially modulate neuropeptide release from distinct sites within a single neuron pair.
Monoamines differentially modulate neuropeptide release from distinct sites within a single neuron pair.
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DOI：
10.1371/journal.pone.0196954
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Komuniecki R
中科院分区：
文献类型：
--
作者：
Clark T;Hapiak V;Oakes M;Mills H;Komuniecki R
单胺和神经肽通常调节相同的行为，但单胺能肽能串扰仍然知之甚少。在秀丽隐杆线虫中，肾上腺素能样配体酪胺（TA）和章鱼胺（OA）需要两个ASI感觉神经元中不同的神经肽子集来抑制伤害性感受。TA选择性地增加nlp-14或nlp-18编码的ASI神经肽分别从ASI轴突的突触/突触周区域或ASI索马体的释放，而OA选择性地增加nlp-9编码的ASI神经肽仅从右侧ASI轴突的突触/突触周区域不对称地释放。预测的编码TA或OA依赖性神经肽的基因的氨基酸前体序列显着不同。然而，这些不同的preprosequence是不足以赋予单胺特异性和额外的N-末端肽编码序列是必需的。总的来说，我们的研究结果表明，TA和OA特异性和差异调节不同的亚细胞内的ASI的神经肽的子集的释放，突出了单胺能/肽能调制的复杂性，即使在一个相对简单的神经系统的动物。
Monoamines and neuropeptides often modulate the same behavior, but monoaminergic-peptidergic crosstalk remains poorly understood. In Caenorhabditis elegans, the adrenergic-like ligands, tyramine (TA) and octopamine (OA) require distinct subsets of neuropeptides in the two ASI sensory neurons to inhibit nociception. TA selectively increases the release of ASI neuropeptides encoded by nlp-14 or nlp-18 from either synaptic/perisynaptic regions of ASI axons or the ASI soma, respectively, and OA selectively increases the release of ASI neuropeptides encoded by nlp-9 asymmetrically, from only the synaptic/perisynaptic region of the right ASI axon. The predicted amino acid preprosequences of genes encoding either TA- or OA-dependent neuropeptides differed markedly. However, these distinct preprosequences were not sufficient to confer monoamine-specificity and additional N-terminal peptide-encoding sequence was required. Collectively, our results demonstrate that TA and OA specifically and differentially modulate the release of distinct subsets of neuropeptides from different subcellular sites within the ASIs, highlighting the complexity of monoaminergic/peptidergic modulation, even in animals with a relatively simple nervous system.
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影响因子：
9.8
作者：
Donnelly JL;Clark CM;Leifer AM;Pirri JK;Haburcak M;Francis MM;Samuel AD;Alkema MJ
通讯作者：
Alkema MJ
影响因子：
29
作者：
Greer, Elisabeth R.;Perez, Carissa L.;Van Gilst, Marc R.;Lee, Brian H.;Ashrafi, Kaveh
通讯作者：
Ashrafi, Kaveh
影响因子：
4.7
作者：
Charbogne P;Kieffer BL;Befort K
通讯作者：
Befort K
DOI：
10.1126/science.1226201
发表时间：
2012-10-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Garrison JL;Macosko EZ;Bernstein S;Pokala N;Albrecht DR;Bargmann CI
通讯作者：
Bargmann CI
影响因子：
64.8
作者：
Euler, T;Detwiler, PB;Denk, W
通讯作者：
Denk, W