Comparison of Antimicrobial-Resistant Escherichia coli Isolates from Urban Raccoons and Domestic Dogs
Comparison of Antimicrobial-Resistant Escherichia coli Isolates from Urban Raccoons and Domestic Dogs
复制标题
从城市浣熊和家犬中分离出的耐药大肠杆菌的比较
DOI：
10.1128/aem.00484-21
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发表时间：
2021
影响因子：
4.4
通讯作者：
Craft, Meggan E.
中科院分区：
文献类型：
--
作者：
Worsley-Tonks, Katherine E.;Gehrt, Stanley D.;Miller, Elizabeth A.;Singer, Randall S.;Bender, Jeff B.;Forester, James D.;McKenzie, Shane C.;Travis, Dominic A.;Johnson, Timothy J.;Craft, Meggan E.
野生动物可以通过多种途径暴露于抗微生物耐药细菌（ARB）。与家畜的空间重叠是一个突出的接触途径。然而，大多数关于野生动物-家养动物界面的研究都集中在家畜上，而对野生动物-伴侣动物界面知之甚少。在这里，我们调查了流行和超广谱头孢菌素耐药（ESC-R）大肠杆菌从浣熊（Procyon lotor）和家犬（犬狼疮familiaris）在大都会地区的芝加哥，IL，美国的系统发育相关性。为了评估与狗的空间重叠的潜在重要性，我们探索了在公园采样的浣熊（即，公园的人和狗可以进入）的患病率和ESC-RE的系统发育相关性不同。大肠杆菌对在私人公园采样的浣熊的感染（即，人和狗不能进入的公园）。浣熊的ESC-RE患病率显著高于其他动物。大肠杆菌感染率（56.9%）高于犬（16.5%）。然而，丰富的ESC-R E.大肠杆菌没有变化的宿主物种。此外，基于核心单核苷酸多态性（SNP）的系统发育分析显示，分离株不按宿主物种聚类，并且在某些情况下显示出高度的相似性（即，差异小于20个核心SNP）。空间重叠分析表明ESC-R E.大肠杆菌在公共公园的浣熊身上比在私人公园的浣熊身上更容易分离出来，但只限于位于芝加哥郊区的公园，而不是市区。这就是说，ESC-R E。从浣熊身上分离的大肠杆菌并不因原产地而遗传聚集。我们的研究结果表明，家犬和城市/郊区浣熊可以有各种各样的ARB，其中一些显示出高度的遗传相关性（即，差异小于20个核心SNP）。鉴于患病率的差异，家犬不太可能成为城市化地区中型食肉动物的重要暴露源。重要信息抗微生物耐药菌（ARB）已在地球仪的许多野生动物物种中检测到，这可能对人类和动物健康产生重要影响。野生动物可以通过多种途径接触ARB，包括通过与家畜的空间重叠。然而，与家畜的界面主要是为牲畜探索的，对野生动物和伴侣动物之间的界面知之甚少。我们的工作表明，城市和郊区的野生动物可以有类似的ARB当地的家犬，但当地的狗不太可能是一个直接的接触源，为城市适应的野生动物。这一发现很重要，因为它强调了将野生动物纳入抗生素耐药性监测工作的必要性，并调查某些城市野生动物物种是否可以作为伴侣动物和间接人类接触的额外流行病学途径。
Wildlife can be exposed to antimicrobial-resistant bacteria (ARB) via multiple pathways. Spatial overlap with domestic animals is a prominent exposure pathway. However, most studies of wildlife-domestic animal interfaces have focused on livestock and little is known about the wildlife-companion animal interface. Here, we investigated the prevalence and phylogenetic relatedness of extended-spectrum cephalosporin-resistant (ESC-R) Escherichia coli from raccoons (Procyon lotor) and domestic dogs (Canis lupus familiaris) in the metropolitan area of Chicago, IL, USA. To assess the potential importance of spatial overlap with dogs, we explored whether raccoons sampled at public parks (i.e., parks where people and dogs could enter) differed in prevalence and phylogenetic relatedness of ESC-R E. coli to raccoons sampled at private parks (i.e., parks where people and dogs could not enter). Raccoons had a significantly higher prevalence of ESC-R E. coli (56.9%) than dogs (16.5%). However, the richness of ESC-R E. coli did not vary by host species. Further, core single-nucleotide polymorphism (SNP)-based phylogenetic analyses revealed that isolates did not cluster by host species, and in some cases displayed a high degree of similarity (i.e., differed by less than 20 core SNPs). Spatial overlap analyses revealed that ESC-R E. coli were more likely to be isolated from raccoons at public parks than raccoons at private parks, but only for parks located in suburban areas of Chicago, not urban areas. That said, ESC-R E. coli isolated from raccoons did not genetically cluster by park of origin. Our findings suggest that domestic dogs and urban/suburban raccoons can have a diverse range of ARB, some of which display a high degree of genetic relatedness (i.e., differ by less than 20 core SNPs). Given the differences in prevalence, domestic dogs are unlikely to be an important source of exposure for mesocarnivores in urbanized areas.IMPORTANCEAntimicrobial-resistant bacteria (ARB) have been detected in numerous wildlife species across the globe, which may have important implications for human and animal health. Wildlife can be exposed to ARB via numerous pathways, including via spatial overlap with domestic animals. However, the interface with domestic animals has mostly been explored for livestock and little is known about the interface between wild animals and companion animals. Our work suggests that urban and suburban wildlife can have similar ARB to local domestic dogs, but local dogs are unlikely to be a direct source of exposure for urban-adapted wildlife. This finding is important because it underscores the need to incorporate wildlife into antimicrobial resistance surveillance efforts, and to investigate whether certain urban wildlife species could act as additional epidemiological pathways of exposure for companion animals, and indirectly for humans.
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