Tissue-specific transcriptomics in the field cricket Teleogryllus oceanicus.
Tissue-specific transcriptomics in the field cricket Teleogryllus oceanicus.
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DOI：
10.1534/g3.112.004341
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发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
Simmons LW
中科院分区：
文献类型：
--
作者：
Bailey NW;Veltsos P;Tan YF;Millar AH;Ritchie MG;Simmons LW
蟋蟀（蟋蟀科）经常被用于行为遗传学、性选择和性冲突的研究，但还没有不同组织类型之间转录组差异的研究。我们评估睾丸，附属腺，其余的全身制剂从男性的领域板球，Teleogryllus oceanicus之间的转录组变化。在Roche 454平台上对来自每个组织的非标准化cDNA文库进行测序，并使用睾丸、附属腺和全身制备物构建主组装体。将总共940，200个读段组装成41，962个重叠群，向其中添加36，856个单例（未组装成重叠群的读段）以提供用于注释分析的总共78，818个序列。总共有59，072个序列（75%）是三种组织中唯一的。睾丸组织特异性序列所占比例最大（62.6%），其次是全身组织（56.43%）和副腺组织（44.16%）。我们测试的假设，表达基因产物的组织，预计将迅速发展的结果，性选择睾丸和附属腺，将产生一个较小比例的BLASTx匹配的同源基因的模式生物果蝇相比，全身组织。睾丸和附腺中唯一表达的序列与注释的D.与一般身体组织中的基因相比，黑腹动物的基因。这些结果与经验证据相一致，即睾丸和附属腺组织中表达的基因是快速进化的选择目标。
Field crickets (family Gryllidae) frequently are used in studies of behavioral genetics, sexual selection, and sexual conflict, but there have been no studies of transcriptomic differences among different tissue types. We evaluated transcriptome variation among testis, accessory gland, and the remaining whole-body preparations from males of the field cricket, Teleogryllus oceanicus. Non-normalized cDNA libraries from each tissue were sequenced on the Roche 454 platform, and a master assembly was constructed using testis, accessory gland, and whole-body preparations. A total of 940,200 reads were assembled into 41,962 contigs, to which 36,856 singletons (reads not assembled into a contig) were added to provide a total of 78,818 sequences used in annotation analysis. A total of 59,072 sequences (75%) were unique to one of the three tissues. Testis tissue had the greatest proportion of tissue-specific sequences (62.6%), followed by general body (56.43%) and accessory gland tissue (44.16%). We tested the hypothesis that tissues expressing gene products expected to evolve rapidly as a result of sexual selection—testis and accessory gland—would yield a smaller proportion of BLASTx matches to homologous genes in the model organism Drosophila melanogaster compared with whole-body tissue. Uniquely expressed sequences in both testis and accessory gland showed a significantly lower rate of matching to annotated D. melanogaster genes compared with those from general body tissue. These results correspond with empirical evidence that genes expressed in testis and accessory gland tissue are rapidly evolving targets of selection.
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