Studies on reproductive characteristics of an interspecific chimera between Brassica juncea and Brassica oleracea
Studies on reproductive characteristics of an interspecific chimera between Brassica juncea and Brassica oleracea
复制标题
芥菜与甘蓝种间嵌合体繁殖特性的研究
DOI：
10.1007/s11240-010-9822-5
复制
发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
Chen, Li-Ping
中科院分区：
文献类型：
--
作者：
Wang, Yan;Cheng, Qi;Zhu, Xue-Yun;Chen, Li-Ping
以红甘蓝和榨菜为材料，研究了其周缘嵌合体的生殖过程。在嵌合体TCC的茎顶端分生组织（L1-L2-L3，L1为茎顶端分生组织的最外层，L2为中间层，L3为最内层; T =榨菜，C =红甘蓝）中，最外层来源于榨菜，而中间层和最内层来源于红甘蓝。在本研究中，位移的L1细胞层被发现导致不育植物。TCC的L2层花粉生活力明显低于红甘蓝，自花受精胚早期败育。胚珠的解剖学观察表明，细胞化胚乳在球胚期部分发育并过早退化。对TCC自交胚进行了胚珠培养，结果表明：授粉后16 d的胚珠在MS +3%蔗糖、0.2%活性炭、400 mg/l谷氨酰胺和水解酪蛋白的培养基上可以得到挽救。形态学、染色体和分子生物学鉴定一致表明，TCC的自交后代是红甘蓝。这些研究表明，异源L1细胞层极大地影响了植物的生殖特性，虽然它不直接参与配子发生和胚胎个体发生。
Periclinal chimera plants of red cabbage and tuber mustard were studied for investigating reproductive processes. In the shoot apical meristem of chimera TCC (L1-L2-L3, L1 being the outermost layer of shoot apical meristem, L2 the middle layer, L3 the innermost layer; T = tuber mustard, C = red cabbage), the outermost layer was derived from tuber mustard, while the middle and the innermost layers were from red cabbage. In the present study, displacement of the L1 cell layer was found to result in infertile plants. The viability of TCC’s pollen from the L2 layer became significantly lower than that of red cabbage, and the self-pollinated embryo aborted at an early stage. Anatomical observation of ovules showed that the cellularized endosperm developed partially and degraded prematurely at the globular-embryo stage. Ovule culture was carried out to rescue the selfed embryos of TCC, and the results showed that ovules at 16 days after pollination could be rescued on Murashige and Skoog (MS) medium supplied with 3% sucrose, 0.2% active carbon, 400 mg/l glutamine, and casein hydrolysate. Morphological, chromosomal, and molecular identifications consistently showed that the selfed progeny of TCC was red cabbage. These investigations indicated that the heterologous L1 cell layer greatly influenced the reproductive characteristics of the plants, although it did not participate directly in gametogenesis and embryo ontogenesis.
登录
查看更多内容
DOI：
10.1007/s11240-010-9686-8
发表时间：
2010-02
期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子：
--
作者：
Xue-Yun Zhu;Shijie Chai;Li-ping Chen;Mingfang Zhang;Jingquan Yu
通讯作者：
Xue-Yun Zhu;Shijie Chai;Li-ping Chen;Mingfang Zhang;Jingquan Yu
影响因子：
2
作者：
Jiangsheng Wu;Zhaohong Li;L. Yi;Han Liu;T. Fu
通讯作者：
Jiangsheng Wu;Zhaohong Li;L. Yi;Han Liu;T. Fu
DOI：
10.5962/bhl.title.5655
发表时间：
2015-08
期刊：
--
影响因子：
--
作者：
C. Wardlaw
通讯作者：
C. Wardlaw
DOI：
10.1016/s1081-1206(10)62947-x
发表时间：
1998
期刊：
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
影响因子：
--
作者：
R. Weber
通讯作者：
R. Weber
影响因子：
2.9
作者：
H. Binding;D. Witt;J. Monzer;G. Mordhorst;R. Kollmann
通讯作者：
H. Binding;D. Witt;J. Monzer;G. Mordhorst;R. Kollmann