Adaptation to Aerobic Environment of Lactobacillus johnsonii/gasseri Strains.
Adaptation to Aerobic Environment of Lactobacillus johnsonii/gasseri Strains.
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DOI：
10.3389/fmicb.2018.00157
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发表时间：
2018
影响因子：
5.2
通讯作者：
Mauriello G
中科院分区：
文献类型：
--
作者：
Maresca D;Zotta T;Mauriello G
氧被认为是影响益生菌存活的主要因素之一，这是由于活性氧（ROS）的作用引起的氧化损伤的诱导。已经表明，乳酸菌的氧化应激抗性强烈依赖于细胞代谢的类型。从发酵到呼吸代谢的转变（通过添加血红素和甲基萘醌，并在氧气的存在下）与生物量的增加，长期生存，和抗氧化酶的生产。这项工作的目的是调查有氧（氧气的存在下）和呼吸（存在下的氧气，血红素，和甲基萘醌）培养的生长动力学，过氧化氢酶的生产，氧的吸收，和氧化应激反应的约氏乳杆菌/加氏菌株以前从婴儿粪便中分离的效果。7株菌在有氧和呼吸条件下均表现出耗氧特性。菌株AL 5在两种生长条件下均表现出过氧化氢酶活性，而菌株AL 3仅在呼吸条件下表现出过氧化氢酶活性。呼吸条件改善了它们对氧化化合物（过氧化氢和ROS发生器）的耐受性，并且进一步显示出有希望的益生菌特征。探索具有益生菌特征的呼吸活性表型对于开发竞争性发酵剂或益生菌培养物可能是非常有用的。
Oxygen is considered one of the main factors affecting probiotic bacteria survival due to the induction of oxidative damages caused by the action of reactive oxygen species (ROS). It has been shown that oxidative stress resistance in lactic acid bacteria is strongly dependent on the type of cell metabolism. Shift from fermentative to respiratory metabolism (through the addition of heme and menaquinone and in presence of oxygen) was associated to increase in biomass, long-term survival, and production of antioxidant enzymes. The aim of this work was to investigate the effect of aerobic (presence of oxygen) and respiratory (presence of oxygen, heme, and menaquinone) cultivation on the growth kinetic, catalase production, oxygen uptake, and oxidative stress response of Lactobacillus johnsonii/gasseri strains previously isolated from infant feces. Seven strains showed to consume oxygen under aerobic and respiratory conditions. The strain AL5 showed a catalase activity in both growth conditions, while AL3 showed this activity only in respiratory condition. Respiratory condition improved their tolerance to oxidative compounds (hydrogen peroxide and ROS generators) and further they showed promising probiotic features. The exploration of respiratory competent phenotypes with probiotic features may be extremely useful for the development of competitive starter or probiotic cultures.
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影响因子：
6.4
作者：
Mills S;Stanton C;Fitzgerald GF;Ross RP
通讯作者：
Ross RP
影响因子：
3.6
作者：
Gaudu, P;Lamberet, G;Gruss, A
通讯作者：
Gruss, A
影响因子：
4.4
作者：
Goffin, Philippe;Muscariello, Lidia;Hols, Pascal
通讯作者：
Hols, Pascal
影响因子：
2.3
作者：
Mitsou, Evdokia K.;Kirtzalidou, Ekaterini;Kyriacou, Adamantini
通讯作者：
Kyriacou, Adamantini
影响因子：
4.4
作者：
KNAUF, HJ;VOGEL, RF;HAMMES, WP
通讯作者：
HAMMES, WP