Paenibacillus oryzisoli sp. nov., isolated from the rhizosphere of rice
Paenibacillus oryzisoli sp. nov., isolated from the rhizosphere of rice
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米类芽孢杆菌 sp.
DOI：
10.1007/s10482-016-0777-3
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
张晓霞
中科院分区：
文献类型：
--
作者：
张晓霞
从水稻根际分离得到一株新细菌，菌株 1ZS3-15T。使用多相方法研究其分类地位。观察到该新菌株为革兰氏染色阳性、能形成孢子、需氧、能运动且呈杆状。基于16S rRNA基因序列的系统发育分析表明，菌株1ZS3-15T在类芽孢杆菌属内恢复。它与类芽孢杆菌 KCTC 13222T（97.9% 相似性）、冷类芽孢杆菌 CCTCC AB 2011150T（96.8%）、类芽孢杆菌 JCM 9068T（96.4%）和类芽孢杆菌 DSM 5051T 密切相关。 (95.5%)。菌株 1ZS3-15T 的脂肪酸谱显示出 anteiso-C15:0 和 iso-C16:0 的优势，支持该菌株归属类芽孢杆菌属。发现主要的甲基萘醌是 MK-7。发现菌株1ZS3-15T的极性脂质谱由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的脂质和两种未鉴定的氨基磷脂组成。细胞壁肽聚糖含有内消旋二氨基庚二酸。根据基因组草图序列，菌株 1ZS3-15T 与密切相关的种 P. pectinilyticus KCTC 13222T 之间的 DNA-DNA 相关性为 24.2 ± 1.0%，菌株之间的平均核苷酸同一性值为 78.9 ± 0.1%，这表明该分离株代表了类芽孢杆菌属的一个新物种。 DNA G+C 含量测定为 45.3 mol%，在类芽孢杆菌属物种报道的范围内。通过基因型、化学分类学和表型分析的表征表明，菌株 1ZS3-15T 代表类芽孢杆菌属的新种，其名称为米类芽孢杆菌 sp。十一月被提议。模式菌株为 1ZS3-15T（= ACCC 19783T = JCM 30487T）。
A novel bacterium, strain 1ZS3-15T, was isolated from rhizosphere of rice. Its taxonomic position was investigated using a polyphasic approach. The novel strain was observed to be Gram-stain positive, spore-forming, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1ZS3-15T was recovered within the genus Paenibacillus. It is closely related to Paenibacillus pectinilyticus KCTC 13222T (97.9 % similarity), Paenibacillus frigoriresistens CCTCC AB 2011150T (96.8 %), Paenibacillus alginolyticus JCM 9068T (96.4 %) and Paenibacillus chondroitinus DSM 5051T (95.5 %). The fatty acid profile of strain 1ZS3-15T, which showed a predominance of anteiso-C15:0 and iso-C16:0, supported the allocation of the strain into the genus Paenibacillus. The predominant menaquinone was found to be MK-7. The polar lipids profile of strain 1ZS3-15T was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and two unidentified aminophospholipids. The cell wall peptidoglycan contains meso-diaminopimelic acid. Based on draft genome sequences, the DNA–DNA relatedness between strain 1ZS3-15T and the closely related species P. pectinilyticus KCTC 13222T are 24.2 ± 1.0 %, and the Average Nucleotide Identity values between the strains are 78.9 ± 0.1 %, which demonstrated that this isolate represents a new species in the genus Paenibacillus. The DNA G+C content was determined to be 45.3 mol%, which is within the range reported for Paenibacillus species. Characterisation by genotypic, chemotaxonomic and phenotypic analysis indicated that strain 1ZS3-15T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus oryzisoli sp. nov. is proposed. The type strain is 1ZS3-15T (= ACCC 19783T = JCM 30487T).
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影响因子：
3
作者：
Jahn K;Winter S;Stoye J;Böcker S
通讯作者：
Böcker S
影响因子：
2.6
作者：
Hyun-Sook. Kim;Sathiyaraj Srinivasan;Sang-Seob Lee
通讯作者：
Hyun-Sook. Kim;Sathiyaraj Srinivasan;Sang-Seob Lee
DOI：
10.1099/ijs.0.002261-0
发表时间：
2009-06
影响因子：
2.8
作者：
Doo-Sang Park;W. Jeong;K. Lee;H. Oh;Byung-Chun Kim;K. Bae;Ho‐Yong Park
通讯作者：
Doo-Sang Park;W. Jeong;K. Lee;H. Oh;Byung-Chun Kim;K. Bae;Ho‐Yong Park
影响因子：
10.7
作者：
TAMURA, K;NEI, M
通讯作者：
NEI, M
影响因子：
10.7
作者：
A. Rzhetsky;M. Nei
通讯作者：
A. Rzhetsky;M. Nei