Two completely linked polymorphisms in the PPARG transcriptional regulatory region significantly affect gene expression and intramuscular fat deposition in the longissimus dorsi muscle of Erhualian pigs.
Two completely linked polymorphisms in the PPARG transcriptional regulatory region significantly affect gene expression and intramuscular fat deposition in the longissimus dorsi muscle of Erhualian pigs.
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PPARG转录调控区两个完全连锁的多态性显着影响二花莲猪背最长肌的基因表达和肌内脂肪沉积。
DOI：
10.1111/age.12025
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发表时间：
2013-08
期刊：
影响因子：
2.4
通讯作者：
Chen, J
中科院分区：
文献类型：
--
作者：
Jiang, Z;Yu, D;Liu, H;Chen, J
本文首次报道了PPARG启动子多态性对二花莲猪背最长肌表达和肌内脂肪（IMF）含量的显著影响。通过对IMF含量差异显著的两个DNA库进行直接测序，扫描PPARG上游转录调控区的多态性位点（P < 0.05）。两个snp （c - 1633c >T和c - 1572g >A）在两组间具有不同的等位基因分布。用PCR-SSCP （single-strand构象多态性）方法对66头二华联猪和24头约克×大白（Y × L）猪进行了这两种snp的基因分型，结果表明，只有T-A和C-G单倍型存在。荧光素酶测定发现T-A型启动子比G-C型启动子在诱导的前脂肪细胞中具有更高的PPARG基因转录活性。此外，二花莲猪的T-A型发病频率远高于Y × L猪（35.6%比8.3%），Y × L猪实际上没有发现T-A/T-A样本。因此，我们得出结论，T-A单倍型PPARG是一种理想的形式，可能有助于二花莲猪相对较高的IMF含量。
This article is the first to report the significant effect of PPARG promoter polymorphisms on the expression and intramuscular fat (IMF) content in the longissimus dorsi (LD) muscle of Erhualian pigs. Polymorphic sites for the PPARG upstream transcriptional regulatory region were scanned by direct sequencing of two DNA pools consisting of individuals with significantly different IMF content (P < 0.05). Two SNPs (c.-1633C>T and c.-1572G>A) were identified, with different allele distribution between these two groups. A total of 66 Erhualian pigs and 24 Yorkshire × Large White (Y × L) pigs were genotyped for these two SNPs using PCR-SSCP (single-strand conformation polymorphism), which exhibited that only the T-A and C-G haplotypes existed. The T-A type promoter was found to have higher activity than G-C type promoter for PPARG gene transcription in the induced preadipocytes using luciferase assay. In addition, the frequency of the T-A type in Erhualian pigs was much higher than that in the Y × L pigs (35.6% vs. 8.3%), and in fact, no T-A/T-A samples were found in Y × L pigs. Therefore, we conclude that the T-A haplotype of PPARG is a desirable form that might contribute to the relatively higher IMF content in Erhualian pigs.
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DOI：
10.5483/bmbrep.2007.40.5.757
发表时间：
2007-09
期刊：
Journal of biochemistry and molecular biology
影响因子：
--
作者：
Seung Hwan Lee;E. Park;Yong-Min Cho;Sung-Kon Kim;Jun-Heon Lee;J. Jeon;C. Lee;S. Im;Sung-Jong Oh;John Mitchell Thompson;D. Yoon
通讯作者：
Seung Hwan Lee;E. Park;Yong-Min Cho;Sung-Kon Kim;Jun-Heon Lee;J. Jeon;C. Lee;S. Im;Sung-Jong Oh;John Mitchell Thompson;D. Yoon
影响因子：
2.7
作者：
HOSHINO, S;KIMURA, A;SASAZUKI, T
通讯作者：
SASAZUKI, T
DOI：
10.1073/pnas.0306743102
发表时间：
2005-04-26
影响因子：
11.1
作者：
Jones, JR;Barrick, C;Magnuson, MA
通讯作者：
Magnuson, MA
DOI：
--
发表时间：
2004-11
期刊：
Yi chuan xue bao = Acta genetica Sinica
影响因子：
--
作者：
Q. Gao;Jun Li;Hong-lin Liu;Lin-Yun Wang;Yin-xue Xu
通讯作者：
Q. Gao;Jun Li;Hong-lin Liu;Lin-Yun Wang;Yin-xue Xu
影响因子：
5.3
作者：
BONNER, JJ;BALLOU, C;FACKENTHAL, DL
通讯作者：
FACKENTHAL, DL