Hedgehog signaling pathway function conserved in Tribolium segmentation.
Hedgehog signaling pathway function conserved in Tribolium segmentation.
复制标题
Hedgehog 信号通路功能在谷盗分段中保守。
DOI：
10.1007/s00427-008-0207-2
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发表时间：
2008-04
影响因子：
2.4
通讯作者：
Brown SJ
中科院分区：
文献类型：
--
作者：
Farzana L;Brown SJ
在果蝇中，节段边界的维持和节段沟的形成依赖于节段极性基因之间的相互作用。无翼和渐变似乎在短胚芽和长胚芽分割中具有相似的作用，但对Hedgehog信号的保守程度知之甚少。在Tribolium基因组计划的配套研究中，我们分析了刺猬、平滑的、补丁的和肘部中断的直系物在Tribolium的分割过程中的表达和功能。它们的表达在果蝇和Tribolium之间基本保守。亲代RNAi分析该途径的阳性调节子(TC-HH、TC-SMO或TC-Ci)导致小的球状角质层，很少或没有节段性凹槽的证据。节段性累加表达在这些胚胎中被启动，但不能维持。在胚带回缩过程中，中枢神经系统保持了无翅独立的渐进化表达，并变得高度致密，这提供了证据，表明这些小的球形胚胎中存在来自每一段的衍生物。另一方面，对负调控因子(TC-PTC)的RNAi分析导致胚胎在胚带伸长过程中可见异位节段凹槽，但在一龄幼虫的角质层中看不到异位节槽。在TC-PTC RNAi胚胎中，这些瞬时沟槽形成于环绕比正常WG结构域更宽的嵌合表达细胞附近。这些结果表明，EN-WG-HH基因回路在功能上是保守的，在Tribolium胚带回缩和沟槽形成过程中维持节段边界，节段极性基因在这种短胚芽昆虫的分段过程中形成了一个强大的遗传调节模块。
In Drosophila, maintenance of parasegmental boundaries and formation of segmental grooves depend on interactions between segment polarity genes. Wingless and Engrailed appear to have similar roles in both short and long germ segmentation, but relatively little is known about the extent to which Hedgehog signaling is conserved. In a companion study to the Tribolium genome project, we analyzed the expression and function of hedgehog, smoothened, patched, and cubitus interruptus orthologs during segmentation in Tribolium. Their expression was largely conserved between Drosophila and Tribolium. Parental RNAi analysis of positive regulators of the pathway (Tc-hh, Tc-smo, or Tc-ci) resulted in small spherical cuticles with little or no evidence of segmental grooves. Segmental Engrailed expression in these embryos was initiated but not maintained. Wingless-independent Engrailed expression in the CNS was maintained and became highly compacted during germ band retraction, providing evidence that derivatives from every segment were present in these small spherical embryos. On the other hand, RNAi analysis of a negative regulator (Tc-ptc) resulted in embryos with ectopic segmental grooves visible during germband elongation but not discernible in the first instar larval cuticles. These transient grooves formed adjacent to Engrailed expressing cells that encircled wider than normal wg domains in the Tc-ptc RNAi embryos. These results suggest that the en–wg–hh gene circuit is functionally conserved in the maintenance of segmental boundaries during germ band retraction and groove formation in Tribolium and that the segment polarity genes form a robust genetic regulatory module in the segmentation of this short germ insect.
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影响因子：
64.8
作者：
DINARDO, S;SHER, E;OFARRELL, PH
通讯作者：
OFARRELL, PH
影响因子：
2.7
作者：
BEEMAN, RW;STUART, JJ;DENELL, RE
通讯作者：
DENELL, RE
DOI：
10.1002/dvg.1020150103
发表时间：
1994-01-01
期刊：
DEVELOPMENTAL GENETICS
影响因子：
--
作者：
BROWN, SJ;PATEL, NH;DENELL, RE
通讯作者：
DENELL, RE
影响因子：
10.5
作者：
Alexandre, C;Jacinto, A;Ingham, PW
通讯作者：
Ingham, PW
影响因子：
2.6
作者：
Alves, G;Limbourg-Bouchon, B;Busson, D
通讯作者：
Busson, D