Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells.
Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells.
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蜂胶对人乳腺癌MCF-7和MDA-MB-231细胞的抗肿瘤活性
DOI：
10.1155/2014/280120
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Hu F
中科院分区：
文献类型：
--
作者：
Xuan H;Li Z;Yan H;Sang Q;Wang K;He Q;Wang Y;Hu F
据报道，中国蜂胶具有抗肿瘤等多种生物活性。通过对人乳腺癌ER(+)细胞和人乳腺癌ER(−)细胞的细胞毒实验，研究了蜂胶乙醇提取物在25、50、100、200 μg/mL4种浓度下的抗癌活性。EECP显示出剂量和时间依赖性的细胞毒效应。此外，还检测了膜联蛋白A7(Annexin A7，ANXA7)、P53、核因子-κB p65(NF-κB p65)、活性氧(ROS)水平和线粒体膜电位。结果表明，体外反搏作用24和48 h后，两种细胞均有明显的凋亡。体外反搏显著增加ANXA7的表达和ROS水平，显著抑制NF-κB p65的表达和线粒体膜电位。EECP对MCF7和MDAMB231细胞中P53表达的影响不同，提示EECP通过诱导细胞凋亡、调节ANXA7、P53和NF-κBp65水平、上调细胞内ROS、降低线粒体膜电位而发挥抗肿瘤作用。有趣的是，EECP对正常人脐静脉内皮细胞(HUVECs)的细胞毒性很小或很小。这些结果表明，EECP是治疗乳腺癌的一种潜在的替代药物。
Chinese propolis has been reported to possess various biological activities such as antitumor. In present study, anticancer activity of ethanol extract of Chinese propolis (EECP) at 25, 50, 100, and 200 μg/mL was explored by testing the cytotoxicity in MCF-7 (human breast cancer ER(+)) and MDA-MB-231 (human breast cancer ER(−)) cells. EECP revealed a dose- and time-dependent cytotoxic effect. Furthermore, annexin A7 (ANXA7), p53, nuclear factor-κB p65 (NF-κB p65), reactive oxygen species (ROS) levels, and mitochondrial membrane potential were investigated. Our data indicated that treatment of EECP for 24 and 48 h induced both cells apoptosis obviously. Exposure to EECP significantly increased ANXA7 expression and ROS level, and NF-κB p65 level and mitochondrial membrane potential were depressed by EECP dramatically. The effects of EECP on p53 level were different in MCF-7 and MDA-MB-231 cells, which indicated that EECP exerted its antitumor effects in MCF-7 and MDA-MB-231 cells by inducing apoptosis, regulating the levels of ANXA7, p53, and NF-κB p65, upregulating intracellular ROS, and decreasing mitochondrial membrane potential. Interestingly, EECP had little or small cytotoxicity on normal human umbilical vein endothelial cells (HUVECs). These results suggest that EECP is a potential alternative agent on breast cancer treatment.
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影响因子：
254.7
作者：
Jemal, Ahmedin;Siegel, Rebecca;Thun, Michael J.
通讯作者：
Thun, Michael J.
影响因子：
--
作者：
Srivastava M;Bubendorf L;Nolan L;Glasman M;Leighton X;Miller G;Fehrle W;Raffeld M;Eidelman O;Kallioniemi OP;Srivastava S;Pollard HB
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作者：
Orsolic, N;Knezevic, AH;Basic, I
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6.1
作者：
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影响因子：
2.2
作者：
Chen, Chia-Nan;Hsiao, Che-Jen;Huang, Wei-Jan
通讯作者：
Huang, Wei-Jan