Ammonium transporter AcAmt mutagenesis uncovers reproductive and physiological defects without impacting olfactory responses to ammonia in the malaria vector mosquito Anopheles coluzzii.
Ammonium transporter AcAmt mutagenesis uncovers reproductive and physiological defects without impacting olfactory responses to ammonia in the malaria vector mosquito Anopheles coluzzii.
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DOI：
10.1016/j.ibmb.2021.103578
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发表时间：
2021-07
影响因子：
3.8
通讯作者：
Zwiebel LJ
中科院分区：
文献类型：
--
作者：
Ye Z;Liu F;Ferguson ST;Baker A;Pitts RJ;Zwiebel LJ
按蚊是疟疾的唯一传播媒介，依靠嗅觉线索寻找宿主，其中来自人类汗液的氨发挥着重要作用。为了研究蚊子嗅觉系统中按蚊铵转运蛋白 (AcAmt) 的功能，我们使用 CRISPR/Cas9 生成了 AcAmt 无效突变体系。与野生型蚊子相比，AcAmt−/− 突变体表现出一系列新的表型，包括交配期间的受精率显着降低和羽化期间的死亡率增加。此外，AcAmt−/− 雄性表现出显着较低的糖消耗，而 AcAmt−/− 雌性和蛹表现出比野生型对应物显着更高的氨水平。令人惊讶的是，之前在果蝇中的研究表明，铵转运蛋白（DmAmt）的突变会导致触角体腔感器中氨反应的急剧减少，而与此相反，在野生型和 AcAmt−/− 雌性之间，一系列外周感觉神经元对氨和其他气味的反应没有观察到显着差异。这些数据支持蚊子中存在新型补偿性氨感应机制，该机制可能是由于氨在寻找宿主和其他行为中的重要性而进化的。
Anopheline mosquitoes are the sole vectors of malaria and rely on olfactory cues for host seeking in which ammonia derived from human sweat plays an essential role. To investigate the function of the Anopheles coluzzii ammonium transporter (AcAmt) in the mosquito olfactory system, we generated an AcAmt null mutant line using CRISPR/Cas9. AcAmt−/− mutants displayed a series of novel phenotypes compared with wild-type mosquitoes including significantly lower insemination rates during mating and increased mortality during eclosion. Furthermore, AcAmt−/− males showed significantly lower sugar consumption while AcAmt−/− females and pupae displayed significantly higher ammonia levels than their wild-type counterparts. Surprisingly, in contrast to previous studies in Drosophila that revealed that the mutation of the ammonium transporter (DmAmt) induces a dramatic reduction of ammonia responses in antennal coeloconic sensilla, no significant differences were observed across a range of peripheral sensory neuron responses to ammonia and other odorants between wild-type and AcAmt−/− females. These data support the existence in mosquitoes of novel compensatory ammonia-sensing mechanisms that are likely to have evolved as a result of the importance of ammonia in host-seeking and other behaviors.
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DOI：
10.1111/j.1460-9568.2007.05786.x
发表时间：
2007-09
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Ghaninia M;Ignell R;Hansson BS
通讯作者：
Hansson BS
影响因子：
3
作者：
Howell PI;Knols BG
通讯作者：
Knols BG
影响因子：
2.8
作者：
Estevez-Lao, Tania Y.;Sigle, Leah T.;Hillyer, Julian F.
通讯作者：
Hillyer, Julian F.
影响因子：
3.2
作者：
Gary, Richard E., Jr.;Cannon, James W., III;Foster, Woodbridge A.
通讯作者：
Foster, Woodbridge A.
影响因子：
2.2
作者：
DENOTTER, CJ;BEHAN, M;MAES, FW
通讯作者：
MAES, FW