Comparison of serum bisphenol A concentrations in mice exposed to bisphenol A through the diet versus oral bolus exposure.
Comparison of serum bisphenol A concentrations in mice exposed to bisphenol A through the diet versus oral bolus exposure.
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DOI：
10.1289/ehp.1003385
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发表时间：
2011-09
影响因子：
10.4
通讯作者：
Rosenfeld CS
中科院分区：
文献类型：
--
作者：
Sieli PT;Jašarevic E;Warzak DA;Mao J;Ellersieck MR;Liao C;Kannan K;Collet SH;Toutain PL;Vom Saal FS;Rosenfeld CS
背景：双酚 A (BPA) 是一种广泛生产的内分泌干扰化学品。饮食是主要的暴露途径，但仅在单次口服推注给药后测量了动物和人类的内部暴露（血清浓度）。目的：我们比较了小鼠口服推注或随意喂养后 24 小时内 BPA 的血清浓度，并评估了饮食暴露引起的 BPA 累积情况。方法：成年雌性小鼠以单次口服推注方式给予[二甲基-d6]-BPA (BPA-d6)（20 mg/kg 体重），或随意饲喂含有 100 mg BPA-d6/kg 饲料重量的饮食，持续 1 周。使用同位素稀释液相色谱结合电喷雾串联质谱分析血清浓度，并在饮食暴露的前 23 小时和 7 天后对暴露组之间进行比较。结果：口服推注组和饮食组分别在 1 小时和 6 小时达到前 24 小时内 BPA-d6 的最大浓度 (Cmax)。尽管吸收相对较低，但饮食暴露小鼠的相对 BPA-d6 生物利用度（未结合的 BPA-d6）高于推注组，这一现象与食物对肝脏首过代谢的抑制作用一致。在持续饮食暴露的小鼠中，第 7 天未结合的 BPA-d6 高于第 1 天。 结论：这是通过饮食接触这种化学物质的动物模型中血清 BPA 浓度的第一份报告。尽管推注 BPA-d6 在 1 小时内达到峰值浓度，但饮食暴露小鼠的 Cmax 延迟了几个小时。然而，与饮食暴露相比，推注给药低估了动物（也可能是人类）的生物可利用血清 BPA 浓度。通过饮食接触是比口服推注接触更自然的持续接触途径，因此可以更好地预测长期接触动物和人类的 BPA 浓度。
Background: Bisphenol A (BPA) is a widely produced endocrine-disrupting chemical. Diet is a primary route of exposure, but internal exposure (serum concentrations) in animals and humans has been measured only after single oral bolus administration. Objective: We compared serum concentrations of BPA over a 24-hr period after oral bolus administration or ad libitum feeding in mice and assessed for buildup with dietary exposure. Methods: Adult female mice were administered [dimethyl-d6]-BPA (BPA-d6) as a single oral bolus (20 mg/kg body weight) or fed a diet containing 100 mg BPA-d6/kg feed weight ad libitum for 1 week. Serum concentrations were analyzed using isotope dilution liquid chromatography coupled with electrospray tandem mass spectrometry and compared between exposure groups over the first 23 hr and after 7 days of dietary exposure. Results: Maximum concentration (Cmax) for BPA-d6 during the first 24 hr was reached at 1 hr and 6 hr for oral bolus and diet groups, respectively. Relative BPA-d6 bioavailability (unconjugated BPA-d6) was higher in diet-exposed mice than in the bolus group despite a relative lower absorption, a phenomenon consistent with an inhibitory effect of food on first-pass hepatic metabolism. In mice with ongoing dietary exposure, unconjugated BPA-d6 was higher on day 7 than on day 1. Conclusions: This is the first report of serum BPA concentrations in an animal model exposed to this chemical via the diet. Although bolus administration of BPA-d6 led to peak concentrations within 1 hr, Cmax for diet-exposed mice was delayed for several hours. However, bolus administration underestimates bioavailable serum BPA concentrations in animals—and presumably humans—than would result from dietary exposure. Exposure via diet is a more natural continuous exposure route than oral bolus exposure and is thus a better predictor of BPA concentrations in chronically exposed animals and humans.
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影响因子：
10.4
作者：
Matsumoto A;Kunugita N;Kitagawa K;Isse T;Oyama T;Foureman GL;Morita M;Kawamoto T
通讯作者：
Kawamoto T
DOI：
10.1016/s0304-4165(02)00418-x
发表时间：
2002-11-14
影响因子：
3
作者：
Sakamoto, H;Yokota, H;Yuasa, A
通讯作者：
Yuasa, A
影响因子：
3.8
作者：
Teeguarden, Justin G.;Calafat, Antonia M.;Graham, Morgan K.
通讯作者：
Graham, Morgan K.
影响因子：
3.5
作者：
Cox, Kimberly H.;Gatewood, Jessica D.;Howeth, Chelsea;Rissman, Emilie F.
通讯作者：
Rissman, Emilie F.
DOI：
10.1073/pnas.0703739104
发表时间：
2007-08-07
影响因子：
11.1
作者：
Dolinoy, Dana C.;Huang, Dale;Jirtle, Randy L.
通讯作者：
Jirtle, Randy L.