On-demand droplet fusion: a strategy for stimulus-responsive biosensing in solution.
On-demand droplet fusion: a strategy for stimulus-responsive biosensing in solution.
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DOI：
10.1021/la502483u
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发表时间：
2014-10-21
期刊：
影响因子：
--
通讯作者：
Goodwin AP
中科院分区：
文献类型：
--
作者：
Mohan P;Noonan PS;Nakatsuka MA;Goodwin AP
报道了一种基于生物化学控制的水包油(O/W)液滴融合作为生物靶标的溶液传感器的新策略。受细胞内SNARE复合体的启发，磷脂、磷脂-聚乙二醇偶联物和胆固醇锚定的寡核苷酸组合稳定了乳剂。在寡核苷酸结合之前，液滴在水介质中是稳定的，但寡核苷酸以拉链的方式杂交被证明启动了液滴的融合。利用染料液滴含量混合的图像分析，研究并优化了熔融专一性作为界面化学的函数。改变锚定的寡核苷酸的方向，使用长链磷脂(C18和C22)，并结合互补的寡核苷酸，完全减缓甚至停止融合。在这些研究的基础上，设计了一种生物标志物凝血酶传感器，该传感器利用适体链的竞争结合，液滴融合随凝血酶的加入而增加，符合简单的结合模型，灵敏度低至100 nM，结果仅需15分钟。未来的努力将集中在利用这种内容混合的机制来促进通过磁阻或化学发光等方式进行高灵敏度的检测。
A novel strategy is reported for biochemically controlled fusion of oil-in-water (O/W) droplets as an in-solution sensor for biological targets. Inspired by the SNARE complex in cells, the emulsions were stabilized by a combination of phospholipids, phospholipid–poly(ethylene glycol) conjugates, and cholesterol-anchored oligonucleotides. Prior to oligonucleotide binding, the droplets were stable in aqueous media, but hybridization of the oligonucleotides in a zipperlike fashion was shown to initiate droplet fusion. Using image analysis of content mixing of dye-loaded droplets, fusion specificity was studied and optimized as a function of interfacial chemistry. Changing the orientation of the anchored oligonucleotides, using long-chain phospholipids (C18 and C22), and binding a complementary oligonucleotide slowed or even halted fusion completely. Based on these studies, a sensor for the biomarker thrombin was designed using competitive binding of aptamer strands, with droplet fusion increasing as a function of thrombin addition in accordance with a simple binding model, with sensitivity down to 100 nM and with results in as little as 15 min. Future efforts will focus on utilizing this mechanism of content mixing to facilitate highly sensitive detection via modalities such as magnetoresistance or chemiluminescence.
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影响因子：
3.5
作者：
Chomas, JE;Dayton, P;Ferrara, K
通讯作者：
Ferrara, K
收缩处双重乳液的破裂
影响因子：
3.4
作者：
Chen, Haosheng;Li, Jiang;Weitz, David A.
通讯作者：
Weitz, David A.
影响因子：
4.2
作者：
Li, Wei;Wang, Kemin;Yang, Xiaohai
通讯作者：
Yang, Xiaohai
影响因子：
15
作者：
Gong, Yun;Luo, Yumei;Bong, Dennis
通讯作者：
Bong, Dennis
DOI：
10.1073/pnas.1214386109
发表时间：
2012-12-11
影响因子：
11.1
作者：
Hadorn, Maik;Boenzli, Eva;Hanczyc, Martin M.
通讯作者：
Hanczyc, Martin M.